biostudies-literatureZhang HResearch on light, green and efficient production technology of foxtail millet, Haiying ZhangCentral Guide Local Science and Technology Development Fund project, suburban special grain and vegetable quality and efficiency of organic drought integrated technology demonstrationBasic Research program of Shanxi ProvinceDoctoral Research project of Shanxi Agricultural Universityearmarked fund for Modern Agro-industry Technology Research Systemearmarked fund for CARS, Xiaoning Cao113https://www.ebi.ac.uk/biostudies/studies/S-EPMC11761630biostudies-literatureUnknown14(1)A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation and column chromatography. Size-Exclusion Chromatography-Multi-Angle Light Scattering-Refractive Index (SEC-MALLS-RI) and high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly purified neutral polysaccharide exclusively composed of glucose as the sugar unit, with a molecular weight of 14.823 kDa. The structural characterization results obtained from gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) spectra suggest that FM-D1 primarily consists of a main chain linked by →4)-α-D-Glc<i>p</i>-(1→ and minor quantities of →4,6)-α-D-Glc<i>p</i>-(1→ to form the main chain, with braAntioxidants (Basel, Switzerland)Structural Characterization of Foxtail Millet (<i>Setaria italica</i>) Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities.PMC11761630YDZJSX2022C017LX202405001No. 2021BQ40 and No. 2023BQ27CARS-06-14.5-A16No. 2024CYJSTX03-23No. 202103021223136Zhang WLiu HLiu XCao XZhang HPeng CSun CfalseStructural Characterization of Foxtail Millet (<i>Setaria italica</i>) Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities.A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation and column chromatography. Size-Exclusion Chromatography-Multi-Angle Light Scattering-Refractive Index (SEC-MALLS-RI) and high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly purified neutral polysaccharide exclusively composed of glucose as the sugar unit, with a molecular weight of 14.823 kDa. The structural characterization results obtained from gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) spectra suggest that FM-D1 primarily consists of a main chain linked by →4)-α-D-Glc<i>p</i>-(1→ and minor quantities of →4,6)-α-D-Glc<i>p</i>-(1→ to form the main chain, with bra2025-01-01T00:00:00Z2025 Jan2025-04-04T22:06:42.569Z2025-04-04T22:06:42.569ZS-EPMC117616303985744610.3390/antiox14010113