biostudies-literatureUnknown15(1)Sarvalkar PDAcoustical properties are essential for understanding the molecular interactions in fluids, as they influence the physicochemical behavior of liquids and determine their suitability for diverse applications. This study investigated the acoustical parameters of silver nanoparticles (Ag NPs), reduced graphene oxide (rGO), and Ag/rGO nanocomposite nanofluids at varying concentrations. Ag NPs and Ag/rGO nanocomposites were synthesized via a Bos taurus indicus (BTI) metabolic waste-assisted method and characterized using advanced techniques, including XRD, TEM, Raman, DLS, zeta potential, and XPS. The synthesized nanocomposites were evaluated for their acoustical, antioxidant, and plant growth-regulatory properties. Acoustical analysis revealed a linear relationship between the nanofluid concenScientific reports3281https://www.ebi.ac.uk/biostudies/studies/S-EPMC11762272biostudies-literatureBio-inspired preparation of Ag NPs, rGO, and Ag/rGO nanocomposites for acoustical, antioxidant, and plant growth regulatory studies.PMC11762272Sarvalkar PDYaul ARRamteke AAJagtap ASPrasad NRSharma KKKfalseBio-inspired preparation of Ag NPs, rGO, and Ag/rGO nanocomposites for acoustical, antioxidant, and plant growth regulatory studies.Acoustical properties are essential for understanding the molecular interactions in fluids, as they influence the physicochemical behavior of liquids and determine their suitability for diverse applications. This study investigated the acoustical parameters of silver nanoparticles (Ag NPs), reduced graphene oxide (rGO), and Ag/rGO nanocomposite nanofluids at varying concentrations. Ag NPs and Ag/rGO nanocomposites were synthesized via a Bos taurus indicus (BTI) metabolic waste-assisted method and characterized using advanced techniques, including XRD, TEM, Raman, DLS, zeta potential, and XPS. The synthesized nanocomposites were evaluated for their acoustical, antioxidant, and plant growth-regulatory properties. Acoustical analysis revealed a linear relationship between the nanofluid concen2025-01-01T00:00:00Z2025 Jan2025-04-21T23:49:18.303Z2025-04-05T19:21:37.007ZS-EPMC117622723986369310.1038/s41598-025-87705-1