{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kassouf MT"],"funding":["Chinese Academy of Medical Sciences (CAMS)","RCUK | MRC | Medical Research Foundation","Medical Research Council","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["1033"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11762767"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Individual enhancers are defined as short genomic regulatory elements, bound by transcription factors, and able to activate cell-specific gene expression at a distance, in an orientation-independent manner. Within mammalian genomes, enhancer-like elements may be found individually or within clusters referred to as locus control regions or super-enhancers (SEs). While these behave similarly to individual enhancers with respect to cell specificity, distribution and distance, their orientation-dependence has not been formally tested. Here, using the α-globin locus as a model, we show that while an individual enhancer works in an orientation-independent manner, the direction of activity of a SE changes with its orientation. When the SE is inverted within its normal chromosomal context, express"],"journal":["Nature communications"],"pubmed_title":["The α-globin super-enhancer acts in an orientation-dependent manner."],"pmcid":["PMC11762767"],"funding_grant_id":["BB/Y008898/1","MR/N00969X/1","109097/Z/15/Z","2018-I2M-2-002","222843/Z/21/Z","MR/T014067/1","219979/Z/19/Z"],"pubmed_authors":["Cornell L","Babbs C","Sousos N","Hughes JR","Francis HS","Telenius J","Suciu MC","Oudelaar M","Kassouf MT","Blayney J","Xella B","Downes DJ","Gosden M","Higgs DR","Sharpe JA","Smith AJH","Larke M","Shen Y","Sloane-Stanley J","Harrold C"],"additional_accession":[]},"is_claimable":false,"name":"The α-globin super-enhancer acts in an orientation-dependent manner.","description":"Individual enhancers are defined as short genomic regulatory elements, bound by transcription factors, and able to activate cell-specific gene expression at a distance, in an orientation-independent manner. Within mammalian genomes, enhancer-like elements may be found individually or within clusters referred to as locus control regions or super-enhancers (SEs). While these behave similarly to individual enhancers with respect to cell specificity, distribution and distance, their orientation-dependence has not been formally tested. Here, using the α-globin locus as a model, we show that while an individual enhancer works in an orientation-independent manner, the direction of activity of a SE changes with its orientation. When the SE is inverted within its normal chromosomal context, express","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-21T23:52:32.505Z","creation":"2025-04-05T19:19:58.54Z"},"accession":"S-EPMC11762767","cross_references":{"pubmed":["39863595"],"doi":["10.1038/s41467-025-56380-1"]}}