biostudies-literatureLi YMunicipal Technology Innovation and Application Development Special Key Projects of Chong-qingScience and Technology Research Program of Chongqing Municipal Education Commission11https://www.ebi.ac.uk/biostudies/studies/S-EPMC11762846biostudies-literatureUnknown14(1)Citrus peel essential oils (CPEOs) have demonstrated substantial medicinal potential for glioblastoma treatment because of their extensive antitumor effects, low potential for drug resistance, and ability to cross the human blood-brain barrier. In this study, the chemical compositions of five CPEOs were analyzed via gas chromatography-mass spectrometry (GC-MS). CCK8 assays were used to evaluate the ability of five CPEOs to inhibit U251 human glioblastoma cells, and XLB and RA were selected for further investigation. Through wound healing assays and cell cycle and apoptosis analyses via flow cytometry, it was revealed that these CPEOs inhibited cell migration, arrested the cell cycle at G1/G0, and induced apoptosis with similar levels of inhibition. After CPEOs treatment, the intracellular Antioxidants (Basel, Switzerland)Essential Oils from Citrus Peels Promote Calcium Overload-Induced Calcicoptosis in U251 Cells.PMC11762846CSTB2022TIAD-KPX0086KJQN202303240Li WXu LJi CLi YWei JYe ZZhou ZfalseEssential Oils from Citrus Peels Promote Calcium Overload-Induced Calcicoptosis in U251 Cells.Citrus peel essential oils (CPEOs) have demonstrated substantial medicinal potential for glioblastoma treatment because of their extensive antitumor effects, low potential for drug resistance, and ability to cross the human blood-brain barrier. In this study, the chemical compositions of five CPEOs were analyzed via gas chromatography-mass spectrometry (GC-MS). CCK8 assays were used to evaluate the ability of five CPEOs to inhibit U251 human glioblastoma cells, and XLB and RA were selected for further investigation. Through wound healing assays and cell cycle and apoptosis analyses via flow cytometry, it was revealed that these CPEOs inhibited cell migration, arrested the cell cycle at G1/G0, and induced apoptosis with similar levels of inhibition. After CPEOs treatment, the intracellular 2024-01-01T00:00:00Z2024 Dec2025-04-04T00:26:16.133Z2025-04-04T00:26:16.133ZS-EPMC117628463985734710.3390/antiox14010011