biostudies-literatureHan JNational Health and Medical Research Council108https://www.ebi.ac.uk/biostudies/studies/S-EPMC11763000biostudies-literatureUnknown14(1)<h4>Background</h4>The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents.<h4>Methods</h4>This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB agents.<h4>Results</h4>Using the combined approach, 11 bioactive compounds were isolated and identified, all exhibiting anti-<i>Mycobacterium bovis</i> BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, and L-O-Lac-L-Val-D-O-Hiv-D-Val, a peptide isolated for the first time from a plant endophyte, demonstrating broad-spectrum antimicrobial activity. Additionally, eAntibiotics (Basel, Switzerland)Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes.PMC117630002017517Liu XLiu MQuinn RJHan JZhang LfalseNuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes.<h4>Background</h4>The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents.<h4>Methods</h4>This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB agents.<h4>Results</h4>Using the combined approach, 11 bioactive compounds were isolated and identified, all exhibiting anti-<i>Mycobacterium bovis</i> BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, and L-O-Lac-L-Val-D-O-Hiv-D-Val, a peptide isolated for the first time from a plant endophyte, demonstrating broad-spectrum antimicrobial activity. Additionally, e2025-01-01T00:00:00Z2025 Jan2025-04-04T22:06:57.822Z2025-04-04T22:06:57.822ZS-EPMC117630003985839310.3390/antibiotics14010108