biostudies-literatureWang Sthe Shandong Province Central Guided Local Science and Technology Development Project for Z Zhuthe Technology System of Modern Agricultural Industry in Shandong Province for L Minthe Start-up Fund for High-level Talents of Qingdao Agricultural University for Z Zhuthe Shandong Province Higher Educational Program for Young Innovation Talents for Z Zhu102https://www.ebi.ac.uk/biostudies/studies/S-EPMC11763317biostudies-literatureUnknown14(1)Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), a mitochondrial-targeted antioxidant, on the post-thaw boar sperm quality during cryopreservation. Boar semen was diluted in a freezing extender containing different concentrations of PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, the sperm motility, viability, acrosome integrity, mitochondrial activity, adenosine triphosphate (ATP) levels, DNA integrity, malondialdehyde (MDA) levels, reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, mitochondrial transcription proteins levels, and fertilization capacity were assessed. The results show that 1000 nM PQQ supplementation tAntioxidants (Basel, Switzerland)Pyrroloquinoline Quinone Improved Boar Sperm Quality via Maintaining Mitochondrial Function During Cryopreservation.PMC117633171121010SDAIT-10-082022KJ170YDZX2021113Min LWang QCao HZhu ZWang SAdetunji AOZhou KfalsePyrroloquinoline Quinone Improved Boar Sperm Quality via Maintaining Mitochondrial Function During Cryopreservation.Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), a mitochondrial-targeted antioxidant, on the post-thaw boar sperm quality during cryopreservation. Boar semen was diluted in a freezing extender containing different concentrations of PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, the sperm motility, viability, acrosome integrity, mitochondrial activity, adenosine triphosphate (ATP) levels, DNA integrity, malondialdehyde (MDA) levels, reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, mitochondrial transcription proteins levels, and fertilization capacity were assessed. The results show that 1000 nM PQQ supplementation t2025-01-01T00:00:00Z2025 Jan2025-04-18T12:48:49.962Z2025-04-06T22:10:08.695ZS-EPMC117633173985743610.3390/antiox14010102