biostudies-literatureUnknown15(1)Mouawad LA sensitive and reliable electrochemical biosensor for the detection of benzalkonium chloride (BAC) and didecyldimethylammonium chloride (DDAC), the most commonly used disinfectant biocides in the agri-food industry, is described. Acetylcholinesterase from <i>Drosophila melanogaster</i> (DM AChE) and butyrylcholinesterase from horse serum (BChE) were immobilized by entrapment in a photocrosslinkable polymer on the surface of carbon screen-printed electrodes. Preliminary tests conducted in phosphate buffer showed limits of detection (LODs) of 0.26 µM for BAC using the BChE-based sensor and 0.04 µM for DDAC using the DM AChE sensor. These performances comply with the European regulation for dairy products, which sets a maximum allowable concentration of 0.28 µM for biocides. However, when teBiosensors26https://www.ebi.ac.uk/biostudies/studies/S-EPMC11763983biostudies-literatureEnhancing Biocide Safety of Milk Using Biosensors Based on Cholinesterase Inhibition.PMC11763983Catanante GNoguer TMouawad LIstamboulie GfalseEnhancing Biocide Safety of Milk Using Biosensors Based on Cholinesterase Inhibition.A sensitive and reliable electrochemical biosensor for the detection of benzalkonium chloride (BAC) and didecyldimethylammonium chloride (DDAC), the most commonly used disinfectant biocides in the agri-food industry, is described. Acetylcholinesterase from <i>Drosophila melanogaster</i> (DM AChE) and butyrylcholinesterase from horse serum (BChE) were immobilized by entrapment in a photocrosslinkable polymer on the surface of carbon screen-printed electrodes. Preliminary tests conducted in phosphate buffer showed limits of detection (LODs) of 0.26 µM for BAC using the BChE-based sensor and 0.04 µM for DDAC using the DM AChE sensor. These performances comply with the European regulation for dairy products, which sets a maximum allowable concentration of 0.28 µM for biocides. However, when te2025-01-01T00:00:00Z2025 Jan2025-04-04T22:07:48.535Z2025-04-04T22:07:48.535ZS-EPMC117639833985207710.3390/bios15010026