biostudies-literatureWang JChina Postdoctoral Science Foundation582https://www.ebi.ac.uk/biostudies/studies/S-EPMC11765360biostudies-literatureUnknown26(2)The accumulation of aniline in the natural environment poses a potential threat to crops, and thus, investigating the effects of aniline on plants holds practical implications for agricultural engineering and its affiliated industries. This study combined physiological, transcriptomic, and metabolomic methods to investigate the growth status and molecular-level response mechanisms of rice under stress from varying concentrations of aniline. At a concentration of 1 mg/L, aniline exhibited a slight growth-promoting effect on rice. However, higher concentrations of aniline significantly inhibited rice growth and even caused notable damage to the rice seedlings. Physiological data indicated that under aniline stress, the membrane of rice underwent oxidative damage. Furthermore, when the concenInternational journal of molecular sciencesIntegrated Physiological, Transcriptomic and Metabolomic Analyses of the Response of Rice to Aniline Toxicity.PMC11765360No. 2021MD703824Zhang WLiu LWang RWang DGuo CGuo RWang JYin ZfalseIntegrated Physiological, Transcriptomic and Metabolomic Analyses of the Response of Rice to Aniline Toxicity.The accumulation of aniline in the natural environment poses a potential threat to crops, and thus, investigating the effects of aniline on plants holds practical implications for agricultural engineering and its affiliated industries. This study combined physiological, transcriptomic, and metabolomic methods to investigate the growth status and molecular-level response mechanisms of rice under stress from varying concentrations of aniline. At a concentration of 1 mg/L, aniline exhibited a slight growth-promoting effect on rice. However, higher concentrations of aniline significantly inhibited rice growth and even caused notable damage to the rice seedlings. Physiological data indicated that under aniline stress, the membrane of rice underwent oxidative damage. Furthermore, when the concen2025-01-01T00:00:00Z2025 Jan2025-04-19T18:23:07.985Z2025-04-19T18:23:07.985ZS-EPMC117653603985929710.3390/ijms26020582