biostudies-literatureWang LGaoyang QuYugang Wang732https://www.ebi.ac.uk/biostudies/studies/S-EPMC11765633biostudies-literatureUnknown26(2)Leaf shape is an important determinant of photosynthesis, yield and quality in plants. In this study, we obtained a curled leaf mutant, <i>cl1</i>, from an ethyl methanesulfonate (EMS)-induced mutagenesis population. It was designated the <i>Brcl1</i>^YS locus. Bulk segregant RNA sequencing combined with recombinant screening identified the candidate interval responsible for <i>Brcl1</i>^YS in a 97.5 kb region on chromosome A02. Twelve genes were identified within the candidate region. Sequence differences and co-separation verification confirmed that <i>BraA02g017030.3C</i> was the most promising candidate gene underlying the <i>Brcl1</i>^YS locus. It is homologous to <i>Arabidopsis AT1G66350</i> (<i>RGL1</i>), which has been shown to act as a negative regulaInternational journal of molecular sciencesIdentification and Candidate Gene Analysis of &lt;i&gt;Brcl1&lt;/i&gt;, a Novel Gene Confers a Leaf Curled Phenotype in &lt;i&gt;Brassica rapa&lt;/i&gt; L.PMC117656332021JH2/102000032023-BSBA-286Liu HLiu YLiu ZJi RHuang SLi CWang WWang YSun YQu GWang LfalseIdentification and Candidate Gene Analysis of &lt;i&gt;Brcl1&lt;/i&gt;, a Novel Gene Confers a Leaf Curled Phenotype in &lt;i&gt;Brassica rapa&lt;/i&gt; L.Leaf shape is an important determinant of photosynthesis, yield and quality in plants. In this study, we obtained a curled leaf mutant, <i>cl1</i>, from an ethyl methanesulfonate (EMS)-induced mutagenesis population. It was designated the <i>Brcl1</i>^YS locus. Bulk segregant RNA sequencing combined with recombinant screening identified the candidate interval responsible for <i>Brcl1</i>^YS in a 97.5 kb region on chromosome A02. Twelve genes were identified within the candidate region. Sequence differences and co-separation verification confirmed that <i>BraA02g017030.3C</i> was the most promising candidate gene underlying the <i>Brcl1</i>^YS locus. It is homologous to <i>Arabidopsis AT1G66350</i> (<i>RGL1</i>), which has been shown to act as a negative regula2025-01-01T00:00:00Z2025 Jan2025-04-18T14:27:12.547Z2025-04-04T22:06:44.5ZS-EPMC117656333985944710.3390/ijms26020732