biostudies-literatureUnknown11(1)Zhang JMorels (<i>Morchella</i> spp.), as one of the rare macroascomycetes that can be cultivated artificially, possess significant economic and scientific values. Morel cultivation is highly sensitive to elevated temperatures; however, the mechanisms of their response to heat shock remain poorly understood. This study integrated transcriptomic and quantitative proteomic analyses of two <i>M. sextelata</i> strains with different thermotolerance (labeled as strains C and D) under normal (18 °C) and high temperature (28 °C) conditions. From over 9300 transcripts and 5000 proteins, both consistency and heterogeneity were found in response to heat shock between the two strains. Both strains displayed a capacity to maintain cellular homeostasis in response to heat shock through highly expressed cell wJournal of fungi (Basel, Switzerland)76https://www.ebi.ac.uk/biostudies/studies/S-EPMC11766532biostudies-literatureIntegrated Transcriptomic and Proteomic Analyses Reveal Molecular Mechanism of Response to Heat Shock in <i>Morchella sextelata</i>.PMC11766532Li YZhang JZhang YZhou BWang WZhang CMao YLiu WfalseIntegrated Transcriptomic and Proteomic Analyses Reveal Molecular Mechanism of Response to Heat Shock in <i>Morchella sextelata</i>.Morels (<i>Morchella</i> spp.), as one of the rare macroascomycetes that can be cultivated artificially, possess significant economic and scientific values. Morel cultivation is highly sensitive to elevated temperatures; however, the mechanisms of their response to heat shock remain poorly understood. This study integrated transcriptomic and quantitative proteomic analyses of two <i>M. sextelata</i> strains with different thermotolerance (labeled as strains C and D) under normal (18 °C) and high temperature (28 °C) conditions. From over 9300 transcripts and 5000 proteins, both consistency and heterogeneity were found in response to heat shock between the two strains. Both strains displayed a capacity to maintain cellular homeostasis in response to heat shock through highly expressed cell w2025-01-01T00:00:00Z2025 Jan2026-05-19T03:11:20.699Z2025-04-05T19:18:58.367ZS-EPMC117665323985249610.3390/jof11010076