biostudies-literatureXu LShanghai Agriculture Applied Technology Development Program, China68https://www.ebi.ac.uk/biostudies/studies/S-EPMC11767259biostudies-literatureUnknown13(1)Phytopathogenic <i>Sclerotinia minor</i> Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing <i>S. minor</i> Jagger were identified using in vitro antagonistic assays against <i>S. minor</i> Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against <i>S. minor</i> Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from <i>gyrA</i>, <i>rpoB</i>, <i>purH</i>, <i>polC</i>, and <i>groEL</i>, Bac20 and Bac45 were identified as <i>Bacillus velezensis</i> and <i>Bacillus subtilis</i>, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted lMicroorganismsBiocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop.PMC11767259X2021-02-08-00-12-F00760Xu LNicolaisen MZeng RShang QGao PZhang JDai FGao SSong ZfalseBiocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop.Phytopathogenic <i>Sclerotinia minor</i> Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing <i>S. minor</i> Jagger were identified using in vitro antagonistic assays against <i>S. minor</i> Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against <i>S. minor</i> Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from <i>gyrA</i>, <i>rpoB</i>, <i>purH</i>, <i>polC</i>, and <i>groEL</i>, Bac20 and Bac45 were identified as <i>Bacillus velezensis</i> and <i>Bacillus subtilis</i>, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted l2025-01-01T00:00:00Z2025 Jan2026-04-18T03:15:12.403Z2025-04-03T23:23:25.351ZS-EPMC117672593985883610.3390/microorganisms13010068