biostudies-literatureUnknown51Heemskerk T<h4>Background and purpose</h4>Understanding the cellular and molecular effect of proton radiation, particularly the increased DNA damage complexity at the distal end of the Bragg curve, is current topic of investigation. This work aims to study <i>in vitro</i> clonogenic survival and DNA damage foci kinetics of a head and neck squamous cell carcinoma cell line at various positions along a double passively scattered Bragg curve. Complementary <i>in silico</i> studies are conducted to gain insights into the link between cell survival variations, experimentally yielded foci and the number and complexity of double strand breaks (DSBs).<h4>Materials and methods</h4>Proton irradiations are performed at the HollandPTC R&D proton beamline, using a double passively scattered setup. A custom water Clinical and translational radiation oncology100908https://www.ebi.ac.uk/biostudies/studies/S-EPMC11772976biostudies-literaturePosition in proton Bragg curve influences DNA damage complexity and survival in head and neck cancer cells.PMC11772976Groenendijk CChatzipapas KKanaar REssers JHeemskerk TLathouwers Dvan der Wal EBrown JMCRovituso Mvan Burik WfalsePosition in proton Bragg curve influences DNA damage complexity and survival in head and neck cancer cells.<h4>Background and purpose</h4>Understanding the cellular and molecular effect of proton radiation, particularly the increased DNA damage complexity at the distal end of the Bragg curve, is current topic of investigation. This work aims to study <i>in vitro</i> clonogenic survival and DNA damage foci kinetics of a head and neck squamous cell carcinoma cell line at various positions along a double passively scattered Bragg curve. Complementary <i>in silico</i> studies are conducted to gain insights into the link between cell survival variations, experimentally yielded foci and the number and complexity of double strand breaks (DSBs).<h4>Materials and methods</h4>Proton irradiations are performed at the HollandPTC R&D proton beamline, using a double passively scattered setup. A custom water 2025-01-01T00:00:00Z2025 Mar2025-04-05T09:32:42.785Z2025-04-05T09:32:42.785ZS-EPMC117729763987729910.1016/j.ctro.2024.100908