{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu P"],"funding":["MOST | National Natural Science Foundation of China (NSFC)","| Beijing Municipal Natural Science Foundation ()"],"pagination":["e0070324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11774033"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["The widespread use of azole antifungals in agriculture and clinical settings has led to serious drug resistance. Overexpression of the azole drug target 14α-demethylase ERG11 (CYP51) is the most common fungal resistance mechanism. However, the presence of additional regulatory proteins in the transcriptional response of <i>erg11</i> is not yet fully elucidated. In this study, leveraging the identified key promoter region of <i>erg11</i> that controls its response to azoles in <i>Neurospora crassa</i>, we pinpointed a protein, Crf4-3, which harbors a PWWP domain and exerts a positive regulatory influence on azole resistance, as determined by DNA pulldown assays. The removal of Crf4-3 results in heightened sensitivity to azoles while remaining unaffected by other stressors tested. Additional"],"journal":["mSphere"],"pubmed_title":["PWWP domain-containing protein Crf4-3 specifically modulates fungal azole susceptibility by regulating sterol C-14 demethylase ERG11."],"pmcid":["PMC11774033"],"funding_grant_id":["No.32370082","No.32100056","No.5242020"],"pubmed_authors":["Zhou M","Hu C","Li S","Zhang Z","Yu P","Ye S","Zhang L","Sun X"],"additional_accession":[]},"is_claimable":false,"name":"PWWP domain-containing protein Crf4-3 specifically modulates fungal azole susceptibility by regulating sterol C-14 demethylase ERG11.","description":"The widespread use of azole antifungals in agriculture and clinical settings has led to serious drug resistance. Overexpression of the azole drug target 14α-demethylase ERG11 (CYP51) is the most common fungal resistance mechanism. However, the presence of additional regulatory proteins in the transcriptional response of <i>erg11</i> is not yet fully elucidated. In this study, leveraging the identified key promoter region of <i>erg11</i> that controls its response to azoles in <i>Neurospora crassa</i>, we pinpointed a protein, Crf4-3, which harbors a PWWP domain and exerts a positive regulatory influence on azole resistance, as determined by DNA pulldown assays. The removal of Crf4-3 results in heightened sensitivity to azoles while remaining unaffected by other stressors tested. Additional","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-02T10:10:58.498Z","creation":"2025-04-03T23:26:44.54Z"},"accession":"S-EPMC11774033","cross_references":{"pubmed":["39670730"],"doi":["10.1128/msphere.00703-24"]}}