biostudies-literatureSong XNational Outstanding Youth Science Fund Project of National Natural Science Foundation of China1529912https://www.ebi.ac.uk/biostudies/studies/S-EPMC11774880biostudies-literatureUnknown15<h4>Objective</h4>This study aimed to screen the differentially expressed genes (DEGs) of <i>Gossypium hirsutum</i> under UV-B stress and identify the significant pathways based on gene enrichment analysis results.<h4>Methods</h4>In this study, the allotetraploid crop <i>G. hirsutum</i> was used to examine changes in various physiological indexes under UV-B stress, and screened out all DEGs under UV-B stress (16 kJ m^-2 d^-1) based on six leaf transcriptomes. The main enrichment pathways of DEGs were analyzed according to gene annotation. Finally, we predicted the regulatory genes of phenylpropanoid pathway under UV-B stress by co-expression network analysis, and selected <i>GhMYB4</i> for verification.<h4>Results</h4>Gene annotation analysis revealed that DEGs were preFrontiers in plant scienceIdentification and characteristics of differentially expressed genes under UV-B stress in <i>Gossypium hirsutum</i>.PMC1177488032070246Zhu YBao YSong XfalseIdentification and characteristics of differentially expressed genes under UV-B stress in <i>Gossypium hirsutum</i>.<h4>Objective</h4>This study aimed to screen the differentially expressed genes (DEGs) of <i>Gossypium hirsutum</i> under UV-B stress and identify the significant pathways based on gene enrichment analysis results.<h4>Methods</h4>In this study, the allotetraploid crop <i>G. hirsutum</i> was used to examine changes in various physiological indexes under UV-B stress, and screened out all DEGs under UV-B stress (16 kJ m^-2 d^-1) based on six leaf transcriptomes. The main enrichment pathways of DEGs were analyzed according to gene annotation. Finally, we predicted the regulatory genes of phenylpropanoid pathway under UV-B stress by co-expression network analysis, and selected <i>GhMYB4</i> for verification.<h4>Results</h4>Gene annotation analysis revealed that DEGs were pre2024-01-01T00:00:00Z20242026-05-26T20:00:36.614Z2025-04-05T23:35:46.639ZS-EPMC117748803988173410.3389/fpls.2024.1529912