biostudies-literatureJi Xthe Clinical Research Special Fund of Wu Jieping Medical Foundatione25145https://www.ebi.ac.uk/biostudies/studies/S-EPMC11776495biostudies-literatureUnknown39(2)<h4>Background</h4>To establish a dual immunoassay based on inductively coupled plasma mass spectrometry (ICP-MS) with stable element labeling antibodies for the simultaneous detection of alpha-fetoprotein (AFP) and prostate-specific antigen (PSA) in serum and evaluate its performance and clinical sample validation.<h4>Methods</h4>The immunoassay system based on the double antibody sandwich method was established using magnetic beads as solid-phase carriers and rare earth elements europium (Eu) and samarium (Sm) as element tags. The test conditions were optimized. According to the Clinical Laboratory Standards Institute (CLSI) guidelines and the "Guidelines for Performance Validation of Clinical Chemistry Quantitative Testing Procedures," performance validation was conducted using the estaJournal of clinical laboratory analysisEstablishment and Application of a Dual Immunoassay Method Based on ICP-MS for Stable Element Labeling Antibodies.PMC11776495320.6750.2022-6-17Wu JYang WLiu YJi XZhang JfalseEstablishment and Application of a Dual Immunoassay Method Based on ICP-MS for Stable Element Labeling Antibodies.<h4>Background</h4>To establish a dual immunoassay based on inductively coupled plasma mass spectrometry (ICP-MS) with stable element labeling antibodies for the simultaneous detection of alpha-fetoprotein (AFP) and prostate-specific antigen (PSA) in serum and evaluate its performance and clinical sample validation.<h4>Methods</h4>The immunoassay system based on the double antibody sandwich method was established using magnetic beads as solid-phase carriers and rare earth elements europium (Eu) and samarium (Sm) as element tags. The test conditions were optimized. According to the Clinical Laboratory Standards Institute (CLSI) guidelines and the "Guidelines for Performance Validation of Clinical Chemistry Quantitative Testing Procedures," performance validation was conducted using the esta2025-01-01T00:00:00Z2025 Jan2025-04-05T09:32:47.577Z2025-04-05T09:32:47.577ZS-EPMC117764953973952510.1002/jcla.25145