{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(3)"],"submitter":["Parada Z"],"pubmed_abstract":["Guanine-rich sequences exhibit a high degree of polymorphism and can form single-stranded, Watson-Crick duplex, and four-stranded G-quadruplex structures. These sequences have found a wide range of uses in synthetic biology applications, arising in part from their structural plasticity. High-throughput, low-cost tools for monitoring the folding and unfolding transitions of G-rich sequences would provide an enabling technology for accelerating the prototyping of synthetic biological systems and for accelerating design-build-test cycles. Here, we show that unfolding transitions of a range of G-quadruplex-forming DNA sequences can be monitored in a FRET-like format using DNA sequences that possess only a single dye label, with no quencher. These quencher-free assays can be performed at low co"],"journal":["ACS omega"],"pagination":["3176-3181"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11780409"],"repository":["biostudies-literature"],"pubmed_title":["Quencher-Free Fluorescence Monitoring of G-Quadruplex Folding."],"pmcid":["PMC11780409"],"pubmed_authors":["Adamala KP","Hoog TG","Parada Z","Engelhart AE"],"additional_accession":[]},"is_claimable":false,"name":"Quencher-Free Fluorescence Monitoring of G-Quadruplex Folding.","description":"Guanine-rich sequences exhibit a high degree of polymorphism and can form single-stranded, Watson-Crick duplex, and four-stranded G-quadruplex structures. These sequences have found a wide range of uses in synthetic biology applications, arising in part from their structural plasticity. High-throughput, low-cost tools for monitoring the folding and unfolding transitions of G-rich sequences would provide an enabling technology for accelerating the prototyping of synthetic biological systems and for accelerating design-build-test cycles. Here, we show that unfolding transitions of a range of G-quadruplex-forming DNA sequences can be monitored in a FRET-like format using DNA sequences that possess only a single dye label, with no quencher. These quencher-free assays can be performed at low co","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-03T23:26:39.119Z","creation":"2025-04-03T23:26:39.119Z"},"accession":"S-EPMC11780409","cross_references":{"pubmed":["39895733"],"doi":["10.1021/acsomega.4c10720"]}}