biostudies-literatureUnknown388Wu J<h4>Objective</h4>To evaluate whether the immunomodulatory drug thymosin α1 reduces mortality in adults with sepsis.<h4>Design</h4>Multicentre, double blinded, placebo controlled phase 3 trial.<h4>Setting</h4>22 centres in China, September 2016 to December 2020.<h4>Participants</h4>1106 adults aged 18-85 years with a diagnosis of sepsis according to sepsis-3 criteria and randomly assigned in a 1:1 ratio to receive thymosin α1 (n=552) or placebo (n=554). A stratified block method was used for randomisation, and participants were stratified by age (<60 and ≥60 years) and centre.<h4>Interventions</h4>Subcutaneous injection of thymosin α1 or placebo every 12 hours for seven days unless discontinued owing to discharge from the intensive care unit, death, or withdrawal of consent.<h4>Main outcome measure</h4>The primary outcome was 28 day all cause mortality after randomisation. All analyses were based on a modified intention-to-treat set, including participants who received at least one dose of study drug.<h4>Results</h4>Of 1106 adults with sepsis enrolled in the study, 1089 were included in the modified intention-to-treat analyses (thymosin α1 group n=542, placebo group n=547). 28 day all cause mortality occurred in 127 participants (23.4%) in the thymosin α1 group and 132 (24.1%) in the placebo group (hazard ratio 0.99, 95% confidence interval 0.77 to 1.27; P=0.93 with log-rank test). No secondary or safety outcome differed statistically significantly between the two groups. The prespecified subgroup analysis showed a potential differential effect of thymosin α1 on the primary outcome based on age (<60 years: hazard ratio 1.67, 1.04 to 2.67; ≥60 years: 0.81, 0.61 to 1.09; P for interaction=0.01) and diabetes (diabetes: 0.58, 0.35 to 0.99; no diabetes: 1.16, 0.87 to 1.53; P for interaction=0.04).<h4>Conclusions</h4>This trial found no clear evidence to suggest that thymosin α1 decreases 28 day all cause mortality in adults with sepsis.<h4>Trial registration</h4>ClinicalTrials.gov NCT02867267.BMJ (Clinical research ed.)e082583https://www.ebi.ac.uk/biostudies/studies/S-EPMC11780596biostudies-literatureThe efficacy and safety of thymosin α1 for sepsis (TESTS): multicentre, double blinded, randomised, placebo controlled, phase 3 trial.PMC11780596Wu JLin QHuang CKe LZhang JWang CPeng SHu XLin WWang FGao JLin YHuang LPei FZhang SQiu LShi YOuyang BShang MSun RShang YChen YZhi DWang WWu WShen YWang ZSu WWu XXu LJin XChen MCheng KKLi RZheng WChen EXu JLi WLi YHu CYang ZHe ZKou QLu JZeng JYao CFeng WYang QZhong JDu LDi YZhong MLiu JTESTS study collaborator groupQiang XGong FLiu XZou XLiu YDing WLiu ZZou ZCai CLong YMao WZhou FLi BChi YNie YZhou JMao ZZhou LYan LYan JDuan MZhou QCui WLuo GZhou XZhu DXiao PZhang WHuang TGuan XZhang XTai ZXuan LfalseThe efficacy and safety of thymosin α1 for sepsis (TESTS): multicentre, double blinded, randomised, placebo controlled, phase 3 trial.<h4>Objective</h4>To evaluate whether the immunomodulatory drug thymosin α1 reduces mortality in adults with sepsis.<h4>Design</h4>Multicentre, double blinded, placebo controlled phase 3 trial.<h4>Setting</h4>22 centres in China, September 2016 to December 2020.<h4>Participants</h4>1106 adults aged 18-85 years with a diagnosis of sepsis according to sepsis-3 criteria and randomly assigned in a 1:1 ratio to receive thymosin α1 (n=552) or placebo (n=554). A stratified block method was used for randomisation, and participants were stratified by age (<60 and ≥60 years) and centre.<h4>Interventions</h4>Subcutaneous injection of thymosin α1 or placebo every 12 hours for seven days unless discontinued owing to discharge from the intensive care unit, death, or withdrawal of consent.<h4>Main outcome measure</h4>The primary outcome was 28 day all cause mortality after randomisation. All analyses were based on a modified intention-to-treat set, including participants who received at least one dose of study drug.<h4>Results</h4>Of 1106 adults with sepsis enrolled in the study, 1089 were included in the modified intention-to-treat analyses (thymosin α1 group n=542, placebo group n=547). 28 day all cause mortality occurred in 127 participants (23.4%) in the thymosin α1 group and 132 (24.1%) in the placebo group (hazard ratio 0.99, 95% confidence interval 0.77 to 1.27; P=0.93 with log-rank test). No secondary or safety outcome differed statistically significantly between the two groups. The prespecified subgroup analysis showed a potential differential effect of thymosin α1 on the primary outcome based on age (<60 years: hazard ratio 1.67, 1.04 to 2.67; ≥60 years: 0.81, 0.61 to 1.09; P for interaction=0.01) and diabetes (diabetes: 0.58, 0.35 to 0.99; no diabetes: 1.16, 0.87 to 1.53; P for interaction=0.04).<h4>Conclusions</h4>This trial found no clear evidence to suggest that thymosin α1 decreases 28 day all cause mortality in adults with sepsis.<h4>Trial registration</h4>ClinicalTrials.gov NCT02867267.2025-01-01T00:00:00Z2025 Jan2026-06-02T01:52:29.672Z2025-04-07T04:21:17.812ZS-EPMC117805963981442010.1136/bmj-2024-082583