biostudies-literatureUnknown43(1)Sakai Y<h4>Purpose</h4>Pyrrolidinophenone derivatives (PPs) are amphetamine-like designer drugs containing a pyrrolidine ring, and their adverse effects resemble those of methamphetamine (METH). Microglial activation has been recently suggested as a key event in eliciting the adverse effects against dysfunction of the central nervous system. The aim of this study is to clarify the mechanisms of microglial activation induced by PPs.<h4>Methods</h4>We employed the human microglial cell line HMC3 to assess microglial activation induced by PPs and evaluated the capacities for proliferation and interleukin-6 (IL-6) production that are characteristic features of the activation events.<h4>Results</h4>The WST-1 assay indicated that viability of HMC3 cells was increased by treatment with sublethal concentForensic toxicology142-154https://www.ebi.ac.uk/biostudies/studies/S-EPMC11782452biostudies-literatureα-Pyrrolidinooctanophenone facilitates activation of human microglial cells via ROS/STAT3-dependent pathway.PMC11782452Hattori JSuenami KSakai YJimbo STakayama TNagai AMatsumura TMichiue TMorikawa YIkari AMatsunaga Tfalseα-Pyrrolidinooctanophenone facilitates activation of human microglial cells via ROS/STAT3-dependent pathway.<h4>Purpose</h4>Pyrrolidinophenone derivatives (PPs) are amphetamine-like designer drugs containing a pyrrolidine ring, and their adverse effects resemble those of methamphetamine (METH). Microglial activation has been recently suggested as a key event in eliciting the adverse effects against dysfunction of the central nervous system. The aim of this study is to clarify the mechanisms of microglial activation induced by PPs.<h4>Methods</h4>We employed the human microglial cell line HMC3 to assess microglial activation induced by PPs and evaluated the capacities for proliferation and interleukin-6 (IL-6) production that are characteristic features of the activation events.<h4>Results</h4>The WST-1 assay indicated that viability of HMC3 cells was increased by treatment with sublethal concent2025-01-01T00:00:00Z2025 Jan2025-04-04T14:05:36.535Z2025-04-04T14:05:36.535ZS-EPMC117824523965214810.1007/s11419-024-00708-x