{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Taylor ZA"],"funding":["NIDCR NIH HHS","HHS | NIH | National Institute of Dental and Craniofacial Research (NIDCR)","HHS | NIH | National Institute of Dental and Craniofacial Research"],"pagination":["e0193524"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784104"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["91(1)"],"pubmed_abstract":["Previous work reported that deletion of the Enzyme IIAB subunits (EIIAB<sup>Man</sup> and <i>manL</i>) of the glucose phosphotransferase system (PTS) (glucose-PTS, <i>manLMNO</i>) in <i>Streptococcus sanguinis</i> impacted carbon catabolite repression and bacterial fitness. Here, a single-nucleotide polymorphism in ManN, ManNA91E, produced the unusual phenotype of increased excretion of organic acids and H<sub>2</sub>O<sub>2</sub> yet elevated PTS activities. To characterize the contributions of each component of the glucose-PTS to bacterial fitness, we performed genetic analyses by deleting from <i>S. sanguinis</i> SK36 the entire operon and each EII<sup>Man</sup> subunit individually; and genes encoding the catabolite control protein A (Δ<i>ccpA</i>) and the redox regulator Rex (Δ<i>rex<"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Systematic analysis of the glucose-PTS in &lt;i&gt;Streptococcus sanguinis&lt;/i&gt; highlighted its importance in central metabolism and bacterial fitness."],"pmcid":["PMC11784104"],"funding_grant_id":["T90 DE021990","DE12236","R01 DE012236"],"pubmed_authors":["Taylor ZA","Pham DN","Zeng L"],"additional_accession":[]},"is_claimable":false,"name":"Systematic analysis of the glucose-PTS in &lt;i&gt;Streptococcus sanguinis&lt;/i&gt; highlighted its importance in central metabolism and bacterial fitness.","description":"Previous work reported that deletion of the Enzyme IIAB subunits (EIIAB<sup>Man</sup> and <i>manL</i>) of the glucose phosphotransferase system (PTS) (glucose-PTS, <i>manLMNO</i>) in <i>Streptococcus sanguinis</i> impacted carbon catabolite repression and bacterial fitness. Here, a single-nucleotide polymorphism in ManN, ManNA91E, produced the unusual phenotype of increased excretion of organic acids and H<sub>2</sub>O<sub>2</sub> yet elevated PTS activities. To characterize the contributions of each component of the glucose-PTS to bacterial fitness, we performed genetic analyses by deleting from <i>S. sanguinis</i> SK36 the entire operon and each EII<sup>Man</sup> subunit individually; and genes encoding the catabolite control protein A (Δ<i>ccpA</i>) and the redox regulator Rex (Δ<i>rex<","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-18T16:49:52.893Z","creation":"2025-04-07T04:15:44.295Z"},"accession":"S-EPMC11784104","cross_references":{"pubmed":["39584828"],"doi":["10.1128/aem.01935-24"]}}