biostudies-literatureTaylor ZANIDCR NIH HHSHHS | NIH | National Institute of Dental and Craniofacial Research (NIDCR)HHS | NIH | National Institute of Dental and Craniofacial Researche0193524https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784104biostudies-literatureUnknown91(1)Previous work reported that deletion of the Enzyme IIAB subunits (EIIAB^Man and <i>manL</i>) of the glucose phosphotransferase system (PTS) (glucose-PTS, <i>manLMNO</i>) in <i>Streptococcus sanguinis</i> impacted carbon catabolite repression and bacterial fitness. Here, a single-nucleotide polymorphism in ManN, ManNA91E, produced the unusual phenotype of increased excretion of organic acids and H₂O₂ yet elevated PTS activities. To characterize the contributions of each component of the glucose-PTS to bacterial fitness, we performed genetic analyses by deleting from <i>S. sanguinis</i> SK36 the entire operon and each EII^Man subunit individually; and genes encoding the catabolite control protein A (Δ<i>ccpA</i>) and the redox regulator Rex (Δ<i>rex<Applied and environmental microbiologySystematic analysis of the glucose-PTS in &lt;i&gt;Streptococcus sanguinis&lt;/i&gt; highlighted its importance in central metabolism and bacterial fitness.PMC11784104T90 DE021990DE12236R01 DE012236Taylor ZAPham DNZeng LfalseSystematic analysis of the glucose-PTS in &lt;i&gt;Streptococcus sanguinis&lt;/i&gt; highlighted its importance in central metabolism and bacterial fitness.Previous work reported that deletion of the Enzyme IIAB subunits (EIIAB^Man and <i>manL</i>) of the glucose phosphotransferase system (PTS) (glucose-PTS, <i>manLMNO</i>) in <i>Streptococcus sanguinis</i> impacted carbon catabolite repression and bacterial fitness. Here, a single-nucleotide polymorphism in ManN, ManNA91E, produced the unusual phenotype of increased excretion of organic acids and H₂O₂ yet elevated PTS activities. To characterize the contributions of each component of the glucose-PTS to bacterial fitness, we performed genetic analyses by deleting from <i>S. sanguinis</i> SK36 the entire operon and each EII^Man subunit individually; and genes encoding the catabolite control protein A (Δ<i>ccpA</i>) and the redox regulator Rex (Δ<i>rex<2025-01-01T00:00:00Z2025 Jan2025-04-18T16:49:52.893Z2025-04-07T04:15:44.295ZS-EPMC117841043958482810.1128/aem.01935-24