{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kakizaki M"],"funding":["MEXT | Japan Society for the Promotion of Science","Shionogi Infectious Disease Research Promotion Foundation","MEXT | Japan Society for the Promotion of Science (JSPS)","The Mitsubishi Foundation","Japan Agency for Medical Research and Development","Astellas Foundation for Research on Metabolic Disorders","The Chemo-Sero-Therapeutic Research Institute","Japan Agency for Medical Research and Development (AMED)"],"pagination":["e0185324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784140"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["99(1)"],"pubmed_abstract":["A critical aspect of the mechanism of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is the protease-mediated activation of the viral spike (S) protein. The type II transmembrane serine protease TMPRSS2 is crucial for SARS-CoV-2 infection in lung epithelial Calu-3 cells and murine airways. However, the importance of TMPRSS2 needs to be re-examined because the ability to utilize TMPRSS2 is significantly reduced in the Omicron variants that spread globally. For this purpose, replication profiles of SARS-CoV-2 were analyzed in human respiratory organoids. All tested viruses, including Omicron variants, replicated efficiently in these organoids. Notably, all SARS-CoV-2 strains retained replication ability in TMPRSS2-gene knockout (KO) respiratory organoids, suggesting t"],"journal":["Journal of virology"],"pubmed_title":["The respective roles of TMPRSS2 and cathepsins for SARS-CoV-2 infection in human respiratory organoids."],"pmcid":["PMC11784140"],"funding_grant_id":["JP243fa627001","JP21gm1610005","23K07935","23K17416","JP23jf0126002","JPJSCCA20240006"],"pubmed_authors":["Kakizaki M","Nagata N","Takeda M","Okura T","Hashimoto R","Ryo A","Kitai Y","Shirato K","Yamamoto T","Akahori Y","Katoh H","Takayama K"],"additional_accession":[]},"is_claimable":false,"name":"The respective roles of TMPRSS2 and cathepsins for SARS-CoV-2 infection in human respiratory organoids.","description":"A critical aspect of the mechanism of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is the protease-mediated activation of the viral spike (S) protein. The type II transmembrane serine protease TMPRSS2 is crucial for SARS-CoV-2 infection in lung epithelial Calu-3 cells and murine airways. However, the importance of TMPRSS2 needs to be re-examined because the ability to utilize TMPRSS2 is significantly reduced in the Omicron variants that spread globally. For this purpose, replication profiles of SARS-CoV-2 were analyzed in human respiratory organoids. All tested viruses, including Omicron variants, replicated efficiently in these organoids. Notably, all SARS-CoV-2 strains retained replication ability in TMPRSS2-gene knockout (KO) respiratory organoids, suggesting t","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T23:11:39.495Z","creation":"2025-04-04T23:11:39.495Z"},"accession":"S-EPMC11784140","cross_references":{"pubmed":["39601592"],"doi":["10.1128/jvi.01853-24"]}}