{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zeng J"],"funding":["MOST | National Natural Science Foundation of China (NSFC)"],"pagination":["e0156624"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784378"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["91(1)"],"pubmed_abstract":["<i>Clostridioides difficile</i> is an obligate anaerobic, Gram-positive bacterium that produces toxins. Despite technological progress, conducting gene expression analysis of <i>C. difficile</i> under different conditions continues to be labor-intensive. Therefore, there is a demand for simplified tools to investigate the transcriptional and translational regulation of <i>C. difficile</i>. The cell-free gene expression (CFE) system has demonstrated utility in various applications, including prototyping, protein production, and <i>in vitro</i> screening. In this study, we developed a <i>C. difficile</i> CFE system capable of <i>in vitro</i> transcription and translation (TX-TL) in the presence of oxygen. Through optimization of cell extract preparation and reaction systems, we increased the"],"journal":["Applied and environmental microbiology"],"pubmed_title":["A &lt;i&gt;Clostridioides difficile&lt;/i&gt; cell-free gene expression system for prototyping and gene expression analysis."],"pmcid":["PMC11784378"],"funding_grant_id":["32370029","82061128001"],"pubmed_authors":["Li Y","Liu Z","Li D","Zhang X","Zeng J","Wu C","Dong M","Wen S","Tian G-B","Wang H","Han J","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"A &lt;i&gt;Clostridioides difficile&lt;/i&gt; cell-free gene expression system for prototyping and gene expression analysis.","description":"<i>Clostridioides difficile</i> is an obligate anaerobic, Gram-positive bacterium that produces toxins. Despite technological progress, conducting gene expression analysis of <i>C. difficile</i> under different conditions continues to be labor-intensive. Therefore, there is a demand for simplified tools to investigate the transcriptional and translational regulation of <i>C. difficile</i>. The cell-free gene expression (CFE) system has demonstrated utility in various applications, including prototyping, protein production, and <i>in vitro</i> screening. In this study, we developed a <i>C. difficile</i> CFE system capable of <i>in vitro</i> transcription and translation (TX-TL) in the presence of oxygen. Through optimization of cell extract preparation and reaction systems, we increased the","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T14:04:56.567Z","creation":"2025-04-04T14:04:56.567Z"},"accession":"S-EPMC11784378","cross_references":{"pubmed":["39745467"],"doi":["10.1128/aem.01566-24"]}}