{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Taguchi J"],"pubmed_abstract":["Temporal control of transgenes has advanced biomedical interventions, including in vivo reprogramming, often utilizing the doxycycline (Dox)-mediated Tet-ON system. Here, we developed the Dox-mediated Tet-ON or complementary Tet-OFF counterpart to thoroughly investigate spatial and temporal transgene regulation in adult tissues, revealing inherent limitations and unexpected capabilities of each system. In stark contrast with the Tet-ON system, which was effective only in particular tissues and cell types, primarily epithelial cells, the Tet-OFF system proved capable of gene induction across diverse cell types. Despite the drawback of the Tet-OFF system in inducibility and tunability identified in our study, we demonstrated that use of tetracycline (Tc) effectively addresses these issues, p"],"journal":["Stem cell reports"],"pagination":["102373"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784451"],"repository":["biostudies-literature"],"pubmed_title":["A versatile in vivo platform for reversible control of transgene expression in adult tissues."],"pmcid":["PMC11784451"],"pubmed_authors":["Taguchi J","Ozawa M","Ohta S","Yamamoto T","Yamada Y","Nakasuka F"],"additional_accession":[]},"is_claimable":false,"name":"A versatile in vivo platform for reversible control of transgene expression in adult tissues.","description":"Temporal control of transgenes has advanced biomedical interventions, including in vivo reprogramming, often utilizing the doxycycline (Dox)-mediated Tet-ON system. Here, we developed the Dox-mediated Tet-ON or complementary Tet-OFF counterpart to thoroughly investigate spatial and temporal transgene regulation in adult tissues, revealing inherent limitations and unexpected capabilities of each system. In stark contrast with the Tet-ON system, which was effective only in particular tissues and cell types, primarily epithelial cells, the Tet-OFF system proved capable of gene induction across diverse cell types. Despite the drawback of the Tet-OFF system in inducibility and tunability identified in our study, we demonstrated that use of tetracycline (Tc) effectively addresses these issues, p","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-01T12:56:05.471Z","creation":"2025-04-04T01:13:19.778Z"},"accession":"S-EPMC11784451","cross_references":{"pubmed":["39642874"],"doi":["10.1016/j.stemcr.2024.11.003"]}}