{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao Z"],"funding":["National Key Research and Development Program of China Stem Cell and Translational Research","Guangdong Provincial Natural Science Foundation","National Natural Science Foundation of China","Qingdao Municipal Science and Technology Bureau","National Key Research and Development Program of China","Taishan Scholar Foundation of Shandong Province"],"pagination":["102382"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11784501"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(1)"],"pubmed_abstract":["Definitive endoderm (DE) derived from human pluripotent stem cells (hPSCs) holds great promise for cell-based therapies and drug discovery. However, current DE differentiation methods required undefined components and/or expensive recombinant proteins, limiting their scalable manufacture and clinical use. Homogeneous DE differentiation in defined and recombinant protein-free conditions remains a major challenge. Here, by systematic optimization and high-throughput screening, we report a chemically defined, small-molecule-based defined system that contains only four components (4C), enabling highly efficient and cost-effective DE specification of hPSCs in the absence of recombinant proteins. 4C-induced DE can differentiate into functional hepatocytes, lung epithelium, and pancreatic β cells"],"journal":["Stem cell reports"],"pubmed_title":["Chemically defined and growth factor-free system for highly efficient endoderm induction of human pluripotent stem cells."],"pmcid":["PMC11784501"],"funding_grant_id":["2022A1515011819","32200685","32471160","32430031","tsqn202306271","92268105","23-2-8-smjk-11-nsh","2023YFA1801200"],"pubmed_authors":["En H","Chan WY","Cao N","Gu S","Lu G","Zeng F","Xu H","Zhao Z","Wang J","Nie Y"],"additional_accession":[]},"is_claimable":false,"name":"Chemically defined and growth factor-free system for highly efficient endoderm induction of human pluripotent stem cells.","description":"Definitive endoderm (DE) derived from human pluripotent stem cells (hPSCs) holds great promise for cell-based therapies and drug discovery. However, current DE differentiation methods required undefined components and/or expensive recombinant proteins, limiting their scalable manufacture and clinical use. Homogeneous DE differentiation in defined and recombinant protein-free conditions remains a major challenge. Here, by systematic optimization and high-throughput screening, we report a chemically defined, small-molecule-based defined system that contains only four components (4C), enabling highly efficient and cost-effective DE specification of hPSCs in the absence of recombinant proteins. 4C-induced DE can differentiate into functional hepatocytes, lung epithelium, and pancreatic β cells","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-01T08:59:30.98Z","creation":"2025-04-03T23:26:45.653Z"},"accession":"S-EPMC11784501","cross_references":{"pubmed":["39729989"],"doi":["10.1016/j.stemcr.2024.11.012"]}}