biostudies-literatureInoue YJapanese Society of HematologyResearch Clusters program of Tokushima UniversityBristol Myers Squibb FoundationJapan Society for the Promotion of Science559-563https://www.ebi.ac.uk/biostudies/studies/S-EPMC11786308biostudies-literatureUnknown116(2)Multiple myeloma (MM) cells and osteoclasts (OCs) activate with each other to cause drug resistance. Human Th1-like Vγ9Vδ2 (γδ) T cells, important effectors against tumors, can be expanded and activated ex vivo by the aminobisphosphonate zoledronic acid in combination with IL-2. We previously reported that the expanded γδ T cells effectively targeted and killed OCs as well as MM cells. Because the expanded γδ T cells expressed CD16 on their surface, we investigated the utilization of the expanded γδ T cells for antibody-dependent cellular cytotoxicity (ADCC). Although the expanded γδ T cells alone induced cell death in MM cell lines, the addition of the anti-SLAMF7 monoclonal antibody elotuzumab (ELO) further enhanced their cytotoxic activity only against SLAMF7-expressing MM cell lines anCancer scienceElotuzumab-mediated ADCC with Th1-like Vγ9Vδ2 T cells to disrupt myeloma-osteoclast interaction.PMC1178630821H03111JP23H03101JP17H0510423195220200322K19626JP22K08455JP19K22719Hiasa MMaeda YKagawa KOzaki SOda AEndo IHarada TMaruhashi TFujii SAbe MHara TOura MSumitani RSogabe KTeramachi JTakahashi MMiki HInoue YNakamura STenshin HfalseElotuzumab-mediated ADCC with Th1-like Vγ9Vδ2 T cells to disrupt myeloma-osteoclast interaction.Multiple myeloma (MM) cells and osteoclasts (OCs) activate with each other to cause drug resistance. Human Th1-like Vγ9Vδ2 (γδ) T cells, important effectors against tumors, can be expanded and activated ex vivo by the aminobisphosphonate zoledronic acid in combination with IL-2. We previously reported that the expanded γδ T cells effectively targeted and killed OCs as well as MM cells. Because the expanded γδ T cells expressed CD16 on their surface, we investigated the utilization of the expanded γδ T cells for antibody-dependent cellular cytotoxicity (ADCC). Although the expanded γδ T cells alone induced cell death in MM cell lines, the addition of the anti-SLAMF7 monoclonal antibody elotuzumab (ELO) further enhanced their cytotoxic activity only against SLAMF7-expressing MM cell lines an2025-01-01T00:00:00Z2025 Feb2025-04-03T23:41:52.081Z2025-04-03T23:41:52.081ZS-EPMC117863083955758610.1111/cas.16401