{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou H"],"funding":["the China Postdoctoral Science Foundation","the Basic Research Plan of Jinling Hospital affiliated to Nanjing University"],"pagination":["58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11786355"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(1)"],"pubmed_abstract":["<h4>Introduction</h4>Ionizing radiation (IR) poses a significant threat to male fertility by inducing substantial changes in the testis, yet the mechanisms underlying IR-induced spermatogenesis disorders remain poorly understood, necessitating the development of more effective radioprotective agents.<h4>Methods</h4>We employed Bulk RNA-seq and single-cell RNA-seq (scRNA-seq) on Balb/c mice testes models following IR exposure to assess cellular and transcriptional alterations. Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. The therapeutic potential of NF-κB agonists was investigated in an IR-induced spermatogenesis disorder model.<h4>Results</h4>A 6 Gy I"],"journal":["Cell communication and signaling : CCS"],"pubmed_title":["Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction."],"pmcid":["PMC11786355"],"funding_grant_id":["No. 22JCYYZD9","No. 2023M734291","No. 2023JCYJYB104","No. 2023JCYJYB103"],"pubmed_authors":["Pan W","Xia X","Zhang J","Wu Q","Chen M","Pang Y","Li W","Liu Z","Zhang X","Jiang C","Zhang C","Zhou H","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction.","description":"<h4>Introduction</h4>Ionizing radiation (IR) poses a significant threat to male fertility by inducing substantial changes in the testis, yet the mechanisms underlying IR-induced spermatogenesis disorders remain poorly understood, necessitating the development of more effective radioprotective agents.<h4>Methods</h4>We employed Bulk RNA-seq and single-cell RNA-seq (scRNA-seq) on Balb/c mice testes models following IR exposure to assess cellular and transcriptional alterations. Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. The therapeutic potential of NF-κB agonists was investigated in an IR-induced spermatogenesis disorder model.<h4>Results</h4>A 6 Gy I","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-03T23:58:21.841Z","creation":"2025-04-03T23:58:21.841Z"},"accession":"S-EPMC11786355","cross_references":{"pubmed":["39891142"],"doi":["10.1186/s12964-025-02067-5"]}}