biostudies-literatureZhou Hthe China Postdoctoral Science Foundationthe Basic Research Plan of Jinling Hospital affiliated to Nanjing University58https://www.ebi.ac.uk/biostudies/studies/S-EPMC11786355biostudies-literatureUnknown23(1)<h4>Introduction</h4>Ionizing radiation (IR) poses a significant threat to male fertility by inducing substantial changes in the testis, yet the mechanisms underlying IR-induced spermatogenesis disorders remain poorly understood, necessitating the development of more effective radioprotective agents.<h4>Methods</h4>We employed Bulk RNA-seq and single-cell RNA-seq (scRNA-seq) on Balb/c mice testes models following IR exposure to assess cellular and transcriptional alterations. Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. The therapeutic potential of NF-κB agonists was investigated in an IR-induced spermatogenesis disorder model.<h4>Results</h4>A 6 Gy ICell communication and signaling : CCSIonizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction.PMC11786355No. 22JCYYZD9No. 2023M734291No. 2023JCYJYB104No. 2023JCYJYB103Pan WXia XZhang JWu QChen MPang YLi WLiu ZZhang XJiang CZhang CZhou HXu ZfalseIonizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction.<h4>Introduction</h4>Ionizing radiation (IR) poses a significant threat to male fertility by inducing substantial changes in the testis, yet the mechanisms underlying IR-induced spermatogenesis disorders remain poorly understood, necessitating the development of more effective radioprotective agents.<h4>Methods</h4>We employed Bulk RNA-seq and single-cell RNA-seq (scRNA-seq) on Balb/c mice testes models following IR exposure to assess cellular and transcriptional alterations. Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. The therapeutic potential of NF-κB agonists was investigated in an IR-induced spermatogenesis disorder model.<h4>Results</h4>A 6 Gy I2025-01-01T00:00:00Z2025 Jan2025-04-03T23:58:21.841Z2025-04-03T23:58:21.841ZS-EPMC117863553989114210.1186/s12964-025-02067-5