biostudies-literaturePatarat RNational Research Council of Thailand52https://www.ebi.ac.uk/biostudies/studies/S-EPMC11786397biostudies-literatureUnknown25(1)<h4>Background</h4>Pulmonary fibrosis is characterized by the destruction of normal lung tissue and then replacement by abnormal fibrous tissue, leading to an overall decrease in gas exchange function. The effective treatment for pulmonary fibrosis remains unknown. The upstream pathogenesis of pulmonary fibrosis may involve cellular senescence of the lung tissue. Previously, a new gene therapy technology using Box A of the HMGB1 plasmid (Box A) was used to reverse cellular senescence and cure liver fibrosis in aged rats.<h4>Methods</h4>Here, we show that Box A is a promising medicine for the treatment of lung fibrosis. In a bleomycin-induced pulmonary fibrosis model in the male Wistar rats, Student's t-test and one-way ANOVA were used to compare groups of samples.<h4>Results</h4>Box A effeBMC pulmonary medicineHMGB1 Box A gene therapy to alleviate bleomycin-induced pulmonary fibrosis in rats.PMC11786397P-19-50189Chuaybudda SYasom SPatarat RMutirangura AfalseHMGB1 Box A gene therapy to alleviate bleomycin-induced pulmonary fibrosis in rats.<h4>Background</h4>Pulmonary fibrosis is characterized by the destruction of normal lung tissue and then replacement by abnormal fibrous tissue, leading to an overall decrease in gas exchange function. The effective treatment for pulmonary fibrosis remains unknown. The upstream pathogenesis of pulmonary fibrosis may involve cellular senescence of the lung tissue. Previously, a new gene therapy technology using Box A of the HMGB1 plasmid (Box A) was used to reverse cellular senescence and cure liver fibrosis in aged rats.<h4>Methods</h4>Here, we show that Box A is a promising medicine for the treatment of lung fibrosis. In a bleomycin-induced pulmonary fibrosis model in the male Wistar rats, Student's t-test and one-way ANOVA were used to compare groups of samples.<h4>Results</h4>Box A effe2025-01-01T00:00:00Z2025 Jan2026-06-01T10:09:30.796Z2025-04-03T23:36:42.57ZS-EPMC117863973989107810.1186/s12890-025-03522-2