biostudies-literatureMaitra SU.S. Department of Agriculture, Agricultural Research ServiceU.S. Department of Energy, Office of Science, Biological and Environmental Research Program14https://www.ebi.ac.uk/biostudies/studies/S-EPMC11786580biostudies-literatureUnknown18(1)<h4>Background</h4>Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane-'oilcane' bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we proposBiotechnology for biofuels and bioproductsResourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: 'transgenic oilcane'.PMC11786580DE-SC0018420CRIS Numbers 5010-41000-189-00DDien BEilts KMaitra SCortes-Pena YRJin YSKuanyshev NGuest JSSingh VfalseResourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: 'transgenic oilcane'.<h4>Background</h4>Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane-'oilcane' bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we propos2025-01-01T00:00:00Z2025 Jan2025-04-03T23:41:57.117Z2025-04-03T23:41:57.117ZS-EPMC117865803989119410.1186/s13068-025-02606-9