{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Santini S"],"funding":["European Research Council","Agence Nationale de la Recherche","GRAL","European Union’s Horizon 2020","Chemistry Biology Health (CBH) Graduate School of University Grenoble Alpes","European Union's Horizon 2020"],"pagination":["wraf002"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11788076"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["The microbial sampling of submarine hydrothermal vents remains challenging, with even fewer studies focused on viruses. Here we report what is to our knowledge the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarviridae. The icosahedral particles of this virus are 200 nm in diameter, with an electron-dense core surrounded by an inner membrane. The viral genome of 395 708 bp (33% G + C) has been predicted to encode 473 proteins. However, besides these standard properties, pacmanvirus lostcity was found to be as"],"journal":["The ISME journal"],"pubmed_title":["Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae."],"pmcid":["PMC11788076"],"funding_grant_id":["ANR-10-INBS-08","832601","ANR-17-EURE-0003"],"pubmed_authors":["Coute Y","Belmudes L","Lartigue A","Alempic JM","Legendre M","Brazelton WJ","Lang SQ","Santini S","Claverie JM","Abergel C"],"additional_accession":[]},"is_claimable":false,"name":"Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae.","description":"The microbial sampling of submarine hydrothermal vents remains challenging, with even fewer studies focused on viruses. Here we report what is to our knowledge the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarviridae. The icosahedral particles of this virus are 200 nm in diameter, with an electron-dense core surrounded by an inner membrane. The viral genome of 395 708 bp (33% G + C) has been predicted to encode 473 proteins. However, besides these standard properties, pacmanvirus lostcity was found to be as","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-02T21:07:49.049Z","creation":"2025-04-19T14:09:30.419Z"},"accession":"S-EPMC11788076","cross_references":{"pubmed":["39789911"],"doi":["10.1093/ismejo/wraf002"]}}