biostudies-literatureChen ZShenzhen Basic Research ProgramGuangdong Basic and Applied Basic Research FundsShenzhen Hospital, Southern Medical UniversityNational Natural Science Foundation of ChinaShanghai Rising-Star ProgramNational Key Research and Development Program of Chinae2411272https://www.ebi.ac.uk/biostudies/studies/S-EPMC11791973biostudies-literatureUnknown12(5)Accurate detection of tumor margins is essential for successful cancer surgery. While indocyanine green (ICG)-based near-infrared (NIR) fluorescence (FL) surgical navigation enhances the visual identification of tumor margins, its accuracy remains subjective, underscoring the need for quantitative approaches. In this study, a high spatiotemporal fluorescence lifetime (FLT) imaging technology is developed in the second near-infrared window (NIR-II, 1000-1700 nm) for quantitative tumor margin detection, utilizing folate receptor-targeted ICG nanoprobes (FPH-ICG). FPH-ICG exhibits a significantly prolonged NIR-II FLT (750 ± 7 ps vs 260 ± 3 ps) and increased NIR-II FL brightness (FPH-ICG/ICG = 3.8). In vitro and in vivo studies confirm that FPH-ICG specifically targets folate receptor-α (FRα) Advanced science (Weinheim, Baden-Wurttemberg, Germany)High Spatiotemporal Near-Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins.PMC11791973B2302021823720222023A151514013392159304822278062023YFF07142002022A15151401022023YFF071520082102123821021062022A151514001723QC14001002024A1515030212E3A10510012023A1515010747JCYJ2023080714081904182171958Liao JHu DZheng HSheng ZChen ZBao ZChen JHuang LZheng WGao DfalseHigh Spatiotemporal Near-Infrared II Fluorescence Lifetime Imaging for Quantitative Detection of Clinical Tumor Margins.Accurate detection of tumor margins is essential for successful cancer surgery. While indocyanine green (ICG)-based near-infrared (NIR) fluorescence (FL) surgical navigation enhances the visual identification of tumor margins, its accuracy remains subjective, underscoring the need for quantitative approaches. In this study, a high spatiotemporal fluorescence lifetime (FLT) imaging technology is developed in the second near-infrared window (NIR-II, 1000-1700 nm) for quantitative tumor margin detection, utilizing folate receptor-targeted ICG nanoprobes (FPH-ICG). FPH-ICG exhibits a significantly prolonged NIR-II FLT (750 ± 7 ps vs 260 ± 3 ps) and increased NIR-II FL brightness (FPH-ICG/ICG = 3.8). In vitro and in vivo studies confirm that FPH-ICG specifically targets folate receptor-α (FRα) 2025-01-01T00:00:00Z2025 Feb2026-05-02T14:33:21.494Z2025-04-19T06:02:30.406ZS-EPMC117919733965244710.1002/advs.202411272