{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(2)"],"submitter":["Soni A"],"funding":["Indian Council of Medical Research"],"pubmed_abstract":["<h4>Aim</h4>Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.<h4>Methods</h4>We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the <i>Mycobacterium tuberculosis</i> MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.<h4>Results</h4>We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (<i>p</i> < 0.01) than magneto-ELISA and GeneXpert.<h4>Conclusion</h4>This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens."],"journal":["Future microbiology"],"pagination":["107-115"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11792811"],"repository":["biostudies-literature"],"pubmed_title":["Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay."],"pmcid":["PMC11792811"],"pubmed_authors":["Raj V","Nehra K","Prasad T","Soni A","Mehta PK","Dahiya B","Gahlaut A","Parmar A","Rais A","Sheoran R"],"additional_accession":[]},"is_claimable":false,"name":"Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.","description":"<h4>Aim</h4>Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.<h4>Methods</h4>We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the <i>Mycobacterium tuberculosis</i> MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.<h4>Results</h4>We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (<i>p</i> < 0.01) than magneto-ELISA and GeneXpert.<h4>Conclusion</h4>This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-06-06T03:08:06.91Z","creation":"2026-06-06T03:06:14.172Z"},"accession":"S-EPMC11792811","cross_references":{"pubmed":["39611745"],"doi":["10.1080/17460913.2024.2432179"]}}