biostudies-literatureUnknown20(2)Soni AIndian Council of Medical Research<h4>Aim</h4>Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.<h4>Methods</h4>We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the <i>Mycobacterium tuberculosis</i> MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.<h4>Results</h4>We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (<i>p</i> < 0.01) than magneto-ELISA and GeneXpert.<h4>Conclusion</h4>This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.Future microbiology107-115https://www.ebi.ac.uk/biostudies/studies/S-EPMC11792811biostudies-literatureDetection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.PMC11792811Raj VNehra KPrasad TSoni AMehta PKDahiya BGahlaut AParmar ARais ASheoran RfalseDetection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.<h4>Aim</h4>Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.<h4>Methods</h4>We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the <i>Mycobacterium tuberculosis</i> MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.<h4>Results</h4>We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (<i>p</i> < 0.01) than magneto-ELISA and GeneXpert.<h4>Conclusion</h4>This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.2025-01-01T00:00:00Z2025 Feb2026-06-06T03:08:06.91Z2026-06-06T03:06:14.172ZS-EPMC117928113961174510.1080/17460913.2024.2432179