{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Karlsson L"],"funding":["Swedish Research Council","Heart and Lung Foundation"],"pagination":["e47-e58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11793033"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["231(1)"],"pubmed_abstract":["<h4>Background</h4>Tuberculosis (TB) is among the largest infectious causes of death worldwide, and there is a need for a time- and resource-effective diagnostic methods. In this novel and exploratory study, we show the potential of using buccal swabs to collect human DNA and investigate the DNA methylation (DNAm) signatures as a diagnostic tool for TB.<h4>Methods</h4>Buccal swabs were collected from patients with pulmonary TB (n = 7), TB-exposed persons (n = 7), and controls (n = 9) in Sweden. Using Illumina MethylationEPIC array, the DNAm status was determined.<h4>Results</h4>We identified 5644 significant differentially methylated CpG sites between the patients and controls. Performing the analysis on a validation cohort of samples collected in Kenya and Peru (patients, n = 26; exposed,"],"journal":["The Journal of infectious diseases"],"pubmed_title":["A DNA Methylation Signature From Buccal Swabs to Identify Tuberculosis Infection."],"pmcid":["PMC11793033"],"funding_grant_id":["20220034","2018-04246","20180613","2018-02961"],"pubmed_authors":["Diero L","Tonui R","Karlsson L","Paues J","Lerm M","Espinoza P","Mendez-Aranda M","Ugarte-Gil C","Sayyab S","Gustafsson M","Ohrnberg I","Martinez-Enguita D"],"additional_accession":[]},"is_claimable":false,"name":"A DNA Methylation Signature From Buccal Swabs to Identify Tuberculosis Infection.","description":"<h4>Background</h4>Tuberculosis (TB) is among the largest infectious causes of death worldwide, and there is a need for a time- and resource-effective diagnostic methods. In this novel and exploratory study, we show the potential of using buccal swabs to collect human DNA and investigate the DNA methylation (DNAm) signatures as a diagnostic tool for TB.<h4>Methods</h4>Buccal swabs were collected from patients with pulmonary TB (n = 7), TB-exposed persons (n = 7), and controls (n = 9) in Sweden. Using Illumina MethylationEPIC array, the DNAm status was determined.<h4>Results</h4>We identified 5644 significant differentially methylated CpG sites between the patients and controls. Performing the analysis on a validation cohort of samples collected in Kenya and Peru (patients, n = 26; exposed,","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2025-04-04T07:26:38.487Z","creation":"2025-04-04T07:26:38.487Z"},"accession":"S-EPMC11793033","cross_references":{"pubmed":["38962817"],"doi":["10.1093/infdis/jiae333"]}}