{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hall JN"],"funding":["Wellcome Trust Intermediate Clinical","Sheffield Biomedical Research Centre","National Institute for Health Research (NIHR)","University of Sheffield","National Institute for Health and Care Research","Higher Education Funding Council for England","Wellcome Trust"],"pagination":["e28-e37"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11793063"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["231(1)"],"pubmed_abstract":["<h4>Background</h4>Streptococcus pyogenes-related skin infections are increasingly implicated in the development of rheumatic heart disease (RHD) in lower-resource settings, where they are often associated with scabies. The true prevalence of S pyogenes-related pyoderma may be underestimated by bacterial culture.<h4>Methods</h4>A multiplex quantitative polymerase chain reaction (qPCR) assay for S pyogenes, Staphylococcus aureus, and Sarcoptes scabiei was applied to 250 pyoderma swabs from a cross-sectional study of children aged <5 years in The Gambia. Direct PCR-based emm-typing was used to supplement previous whole genome sequencing (WGS) of cultured isolates.<h4>Results</h4>Pyoderma lesions with S pyogenes increased from 51% (127/250) using culture to 80% (199/250) with qPCR. Compared t"],"journal":["The Journal of infectious diseases"],"pubmed_title":["Molecular Methods Enhance the Detection of Pyoderma-Related Streptococcus pyogenes and emm-Type Distribution in Children."],"pmcid":["PMC11793063"],"funding_grant_id":["NIHR203321","ACF-2022-04-003"],"pubmed_authors":["Hall JN","Armitage EP","Turner CE","Marks M","Bah S","Senghore E","McCarthy JS","Darton TC","de Silva TI","Angyal A","Barry M","Darboe S","Camara J","Keeley AJ","Smeesters P"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Methods Enhance the Detection of Pyoderma-Related Streptococcus pyogenes and emm-Type Distribution in Children.","description":"<h4>Background</h4>Streptococcus pyogenes-related skin infections are increasingly implicated in the development of rheumatic heart disease (RHD) in lower-resource settings, where they are often associated with scabies. The true prevalence of S pyogenes-related pyoderma may be underestimated by bacterial culture.<h4>Methods</h4>A multiplex quantitative polymerase chain reaction (qPCR) assay for S pyogenes, Staphylococcus aureus, and Sarcoptes scabiei was applied to 250 pyoderma swabs from a cross-sectional study of children aged <5 years in The Gambia. Direct PCR-based emm-typing was used to supplement previous whole genome sequencing (WGS) of cultured isolates.<h4>Results</h4>Pyoderma lesions with S pyogenes increased from 51% (127/250) using culture to 80% (199/250) with qPCR. Compared t","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2025-04-26T01:34:40.018Z","creation":"2025-04-06T10:14:56.658Z"},"accession":"S-EPMC11793063","cross_references":{"pubmed":["39008379"],"doi":["10.1093/infdis/jiae359"]}}