biostudies-literatureHall JNWellcome Trust Intermediate ClinicalSheffield Biomedical Research CentreNational Institute for Health Research (NIHR)University of SheffieldNational Institute for Health and Care ResearchHigher Education Funding Council for EnglandWellcome Truste28-e37https://www.ebi.ac.uk/biostudies/studies/S-EPMC11793063biostudies-literatureUnknown231(1)<h4>Background</h4>Streptococcus pyogenes-related skin infections are increasingly implicated in the development of rheumatic heart disease (RHD) in lower-resource settings, where they are often associated with scabies. The true prevalence of S pyogenes-related pyoderma may be underestimated by bacterial culture.<h4>Methods</h4>A multiplex quantitative polymerase chain reaction (qPCR) assay for S pyogenes, Staphylococcus aureus, and Sarcoptes scabiei was applied to 250 pyoderma swabs from a cross-sectional study of children aged <5 years in The Gambia. Direct PCR-based emm-typing was used to supplement previous whole genome sequencing (WGS) of cultured isolates.<h4>Results</h4>Pyoderma lesions with S pyogenes increased from 51% (127/250) using culture to 80% (199/250) with qPCR. Compared tThe Journal of infectious diseasesMolecular Methods Enhance the Detection of Pyoderma-Related Streptococcus pyogenes and emm-Type Distribution in Children.PMC11793063NIHR203321ACF-2022-04-003Hall JNArmitage EPTurner CEMarks MBah SSenghore EMcCarthy JSDarton TCde Silva TIAngyal ABarry MDarboe SCamara JKeeley AJSmeesters PfalseMolecular Methods Enhance the Detection of Pyoderma-Related Streptococcus pyogenes and emm-Type Distribution in Children.<h4>Background</h4>Streptococcus pyogenes-related skin infections are increasingly implicated in the development of rheumatic heart disease (RHD) in lower-resource settings, where they are often associated with scabies. The true prevalence of S pyogenes-related pyoderma may be underestimated by bacterial culture.<h4>Methods</h4>A multiplex quantitative polymerase chain reaction (qPCR) assay for S pyogenes, Staphylococcus aureus, and Sarcoptes scabiei was applied to 250 pyoderma swabs from a cross-sectional study of children aged <5 years in The Gambia. Direct PCR-based emm-typing was used to supplement previous whole genome sequencing (WGS) of cultured isolates.<h4>Results</h4>Pyoderma lesions with S pyogenes increased from 51% (127/250) using culture to 80% (199/250) with qPCR. Compared t2025-01-01T00:00:00Z2025 Feb2025-04-26T01:34:40.018Z2025-04-06T10:14:56.658ZS-EPMC117930633900837910.1093/infdis/jiae359