{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guanzon DA"],"funding":["German Research Foundation","DFG","Katholischer Akademischer Ausländer-Dienst"],"pagination":["gkaf041"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11795201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(3)"],"pubmed_abstract":["Bacterial pathogens, such as Yersinia pseudotuberculosis, encounter temperature fluctuations during host infection and upon return to the environment. These temperature shifts impact RNA structures globally. While previous transcriptome-wide studies have focused on RNA thermometers in the 5'-untranslated region of virulence-related messenger RNAs, our investigation revealed temperature-driven structural rearrangements in the small RNA CyaR (cyclic AMP-activated RNA). At 25°C, CyaR primarily adopts a conformation that occludes its seed region, but transitions to a liberated state at 37°C. By RNA sequencing and in-line probing experiments, we identified the Shine-Dalgarno sequence of ompX as a direct target of CyaR. Interestingly, the ompX transcript itself exhibits RNA thermometer-like prop"],"journal":["Nucleic acids research"],"pubmed_title":["Two temperature-responsive RNAs act in concert: the small RNA CyaR and the mRNA ompX."],"pmcid":["PMC11795201"],"funding_grant_id":["NA 240/14-1","GRK 2341"],"pubmed_authors":["Dietze A","Wimbert A","Roder J","Brandenburg VB","Twittenhoff C","Narberhaus F","Guanzon DA","Scheller D","Pienkoß S"],"additional_accession":[]},"is_claimable":false,"name":"Two temperature-responsive RNAs act in concert: the small RNA CyaR and the mRNA ompX.","description":"Bacterial pathogens, such as Yersinia pseudotuberculosis, encounter temperature fluctuations during host infection and upon return to the environment. These temperature shifts impact RNA structures globally. While previous transcriptome-wide studies have focused on RNA thermometers in the 5'-untranslated region of virulence-related messenger RNAs, our investigation revealed temperature-driven structural rearrangements in the small RNA CyaR (cyclic AMP-activated RNA). At 25°C, CyaR primarily adopts a conformation that occludes its seed region, but transitions to a liberated state at 37°C. By RNA sequencing and in-line probing experiments, we identified the Shine-Dalgarno sequence of ompX as a direct target of CyaR. Interestingly, the ompX transcript itself exhibits RNA thermometer-like prop","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T02:09:17.123Z","creation":"2025-04-04T02:09:17.123Z"},"accession":"S-EPMC11795201","cross_references":{"pubmed":["39907110"],"doi":["10.1093/nar/gkaf041"]}}