{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leitner DR"],"funding":["NIAID NIH HHS","Life Sciences Research Foundation (LSRF)","Howard Hughes Medical Institute (HHMI)","HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)"],"pagination":["e0343324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11796348"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(2)"],"pubmed_abstract":["Bacteria have evolved diverse strategies to ensure survival under nutrient-limited conditions, where rapid energy generation is not achievable. Here, we performed a transposon insertion site sequencing loss-of-function screen to identify <i>Vibrio cholerae</i> genes that promote pathogen fitness in stationary phase. We discovered that the <u>m</u>aintenance of <u>l</u>ipid <u>a</u>symmetry (Mla) pathway, which is crucial for transferring phospholipids from the outer to the inner membrane, is critical for stationary phase fitness. Competition experiments with barcoded and fluorophore labeled wild-type (WT) and <i>mlaE</i> mutant <i>V. cholerae</i> revealed that the Mla pathway promotes re-expansion from 48 h stationary phase cultures. The mutant defect in transitioning out of stationary pha"],"journal":["mBio"],"pubmed_title":["The Mla pathway promotes &lt;i&gt;Vibrio cholerae&lt;/i&gt; re-expansion from stationary phase."],"pmcid":["PMC11796348"],"funding_grant_id":["R37 AI042347","AI-042347","R01 AI042347","Zingl-2024HHMI"],"pubmed_authors":["Waldor MK","Leitner DR","Zingl FG","Zhang H","Morano AA"],"additional_accession":[]},"is_claimable":false,"name":"The Mla pathway promotes &lt;i&gt;Vibrio cholerae&lt;/i&gt; re-expansion from stationary phase.","description":"Bacteria have evolved diverse strategies to ensure survival under nutrient-limited conditions, where rapid energy generation is not achievable. Here, we performed a transposon insertion site sequencing loss-of-function screen to identify <i>Vibrio cholerae</i> genes that promote pathogen fitness in stationary phase. We discovered that the <u>m</u>aintenance of <u>l</u>ipid <u>a</u>symmetry (Mla) pathway, which is crucial for transferring phospholipids from the outer to the inner membrane, is critical for stationary phase fitness. Competition experiments with barcoded and fluorophore labeled wild-type (WT) and <i>mlaE</i> mutant <i>V. cholerae</i> revealed that the Mla pathway promotes re-expansion from 48 h stationary phase cultures. The mutant defect in transitioning out of stationary pha","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2025-04-04T02:10:04.072Z","creation":"2025-04-04T02:10:04.072Z"},"accession":"S-EPMC11796348","cross_references":{"pubmed":["39714184"],"doi":["10.1128/mbio.03433-24"]}}