biostudies-literatureHuang QGuohong XiaoJingqi Chen10561-10583https://www.ebi.ac.uk/biostudies/studies/S-EPMC11796892biostudies-literatureUnknown149(12)CLIC5 encoded protein associates with actin-based cytoskeletal and is increasingly thought to play significant roles in human cancers. We use TCGA and GEO to explore CLIC5 expression differences, mutation and DNA methylation, TMB, MSI, and immune cell infiltration. We verified the mRNA expression of CLIC5 in human ovarian cancer cells by real-time PCR and detected the expression of CLIC5 as well as immune marker genes in ovarian cancer by immunohistochemistry. The pan-cancer analysis showed that CLIC5 is highly expressed in several malignant tumors. In some cancers, CLIC5 expression in tumor samples is associated with poorer overall survival. For example, patients with ovarian cancer with high expression of CLIC5 have a poor prognosis. CLIC5 mutation frequency increased in all tumor types.Journal of cancer research and clinical oncologyA comprehensively prognostic and immunological analysis of chloride intracellular channel protein 5 (CLIC5) in pan-cancer and identification in ovarian cancer.PMC11796892the 2021 Clinical Research Project of the Second Affiliated Hospital of Guangzhou Medical University (2021-LCYJ-03)National Natural Science Foundation of China(81172537, 81272900, 81772828)Huang CPan YHuang JChen JXing YHe MXiao GHuang QTang WLan HLv QWu HfalseA comprehensively prognostic and immunological analysis of chloride intracellular channel protein 5 (CLIC5) in pan-cancer and identification in ovarian cancer.CLIC5 encoded protein associates with actin-based cytoskeletal and is increasingly thought to play significant roles in human cancers. We use TCGA and GEO to explore CLIC5 expression differences, mutation and DNA methylation, TMB, MSI, and immune cell infiltration. We verified the mRNA expression of CLIC5 in human ovarian cancer cells by real-time PCR and detected the expression of CLIC5 as well as immune marker genes in ovarian cancer by immunohistochemistry. The pan-cancer analysis showed that CLIC5 is highly expressed in several malignant tumors. In some cancers, CLIC5 expression in tumor samples is associated with poorer overall survival. For example, patients with ovarian cancer with high expression of CLIC5 have a poor prognosis. CLIC5 mutation frequency increased in all tumor types.2023-01-01T00:00:00Z2023 Sep2026-03-18T13:58:22.98Z2025-08-21T09:53:04.185ZS-EPMC117968923728673410.1007/s00432-023-04927-4