biostudies-literatureLv LShandong Provincial Natural Science FoundationAcademic Promotion Programme of Shandong First Medical UniversityNational Natural Science FoundationChina Postdoctoral Science FoundationKey Research and Development Program of Shandong ProvinceShandong Provincial Engineering Research Center of LymphomaTaishan Scholars Program of Shandong ProvinceTranslational Research Grant of NCRCH12677-12690https://www.ebi.ac.uk/biostudies/studies/S-EPMC11797697biostudies-literatureUnknown149(14)<h4>Purpose</h4>Emerging evidence suggests that pyroptosis plays an essential role in the development and progression of multiple cancers. However, the role of pyroptosis remains elusive in diffuse large B-cell lymphoma (DLBCL).<h4>Methods</h4>The expression profile data of DLBCL and normal samples of pyroptosis-related genes (PRGs) were analyzed, and the clinical characteristics of DLBCL patients were further investigated. A prognostic model was established using LASSO-Cox regression analysis. The expression of these PRGs was validated by qRT-PCR in DLBCL cell lines. Cell proliferation assay and flow cytometry were utilized to explore the impact of pyroptosis inhibitor (disulfiram, DSF) combined with PD1/PD-L1 inhibitor (BMS1166) on DLBCL cell proliferation.<h4>Results</h4>Most PRGs were Journal of cancer research and clinical oncologyIdentification of pyroptosis-related signature and development of a novel prognostic model in diffuse large B-cell lymphoma.PMC11797697No.2021WWB02No.2020ZKMB01ZR2021YQ51No. 2021T1404223No.2018CXGC1213No. 2019QL018No.82070203, No.81770210No.82170189, No.81800194Lv LKong RZhang YWang CWang XZhou XfalseIdentification of pyroptosis-related signature and development of a novel prognostic model in diffuse large B-cell lymphoma.<h4>Purpose</h4>Emerging evidence suggests that pyroptosis plays an essential role in the development and progression of multiple cancers. However, the role of pyroptosis remains elusive in diffuse large B-cell lymphoma (DLBCL).<h4>Methods</h4>The expression profile data of DLBCL and normal samples of pyroptosis-related genes (PRGs) were analyzed, and the clinical characteristics of DLBCL patients were further investigated. A prognostic model was established using LASSO-Cox regression analysis. The expression of these PRGs was validated by qRT-PCR in DLBCL cell lines. Cell proliferation assay and flow cytometry were utilized to explore the impact of pyroptosis inhibitor (disulfiram, DSF) combined with PD1/PD-L1 inhibitor (BMS1166) on DLBCL cell proliferation.<h4>Results</h4>Most PRGs were 2023-01-01T00:00:00Z2023 Nov2026-03-27T16:57:58.349Z2025-09-01T03:05:18.753ZS-EPMC117976973745285110.1007/s00432-023-05018-0