biostudies-literatureHouweling MBrain Tumour CharityThe Brain Tumour CharityKWF Kankerbestrijding297-305https://www.ebi.ac.uk/biostudies/studies/S-EPMC11798055biostudies-literatureUnknown149(1)<h4>Introduction</h4>Glioblastoma (GBM) is an incurable cancer type. New therapeutic options are investigated, including targeting the mitogen-activated protein kinase (MAPK) pathway using MEK inhibitors as radio-sensitizers. In this study, we investigated whether MEK inhibition via PD0325901 leads to radio-sensitization in experimental in vitro and in vivo models of GBM.<h4>Materials and methods</h4>In vitro, GBM8 multicellular spheroids were irradiated with 3 fractions of 2 Gy, during 5 consecutive days of incubation with either PD0325901 or MEK-162. In vivo, we combined PD0325901 with radiotherapy in the GBM8 orthotopic mouse model, tumor growth was measured weekly by bioluminescence imaging and overall survival and toxicity were assessed.<h4>Results</h4>Regrowth and viability of spheroJournal of cancer research and clinical oncologyRadio-sensitizing effect of MEK inhibition in glioblastoma in vitro and in vivo.PMC11798055488097KWF 4874GN-00042911038Heukelom SWesterman BAWurdinger TNoske DHouweling MVerheul HAbdul UKLagerweij TBrahm CKoken PWSminia PHoneywell RPeters GJWedekind LEfalseRadio-sensitizing effect of MEK inhibition in glioblastoma in vitro and in vivo.<h4>Introduction</h4>Glioblastoma (GBM) is an incurable cancer type. New therapeutic options are investigated, including targeting the mitogen-activated protein kinase (MAPK) pathway using MEK inhibitors as radio-sensitizers. In this study, we investigated whether MEK inhibition via PD0325901 leads to radio-sensitization in experimental in vitro and in vivo models of GBM.<h4>Materials and methods</h4>In vitro, GBM8 multicellular spheroids were irradiated with 3 fractions of 2 Gy, during 5 consecutive days of incubation with either PD0325901 or MEK-162. In vivo, we combined PD0325901 with radiotherapy in the GBM8 orthotopic mouse model, tumor growth was measured weekly by bioluminescence imaging and overall survival and toxicity were assessed.<h4>Results</h4>Regrowth and viability of sphero2023-01-01T00:00:00Z2023 Jan2026-03-27T15:50:09.482Z2025-08-27T03:06:40.443ZS-EPMC117980553645104410.1007/s00432-022-04483-3