biostudies-literatureTakamatsu TMEXT | Japan Society for the Promotion of Science (JSPS)120-128https://www.ebi.ac.uk/biostudies/studies/S-EPMC11799345biostudies-literatureUnknown134(2)Male-killing is a microbe-induced reproductive manipulation in invertebrates whereby male hosts are eliminated during development. In the tea tortrix moth Homona magnanima, Osugoroshi viruses 1‒3 (OGVs), belonging to Partitiviridae induce male-killing. The infection patterns of OGVs are diverse; however, how the influence of these patterns of host phenotypes remains largely unknown. Using field-collected larvae, we established a OGV1 and OGV3 double-infection line, in addition to a triple-infection line, and examined the dsRNA segments, purified viral proteins, OGV density, and host phenotypes. PCR analysis demonstrated that the triple-infection line lost one dsRNA segment, whereas the double-infection line lost eight segments, including one RNA-dependent RNA polymerase (RdRp) gene. LC-MS HeredityInfection pattern of male-killing viruses alters phenotypes in the tea tortrix moth Homona magnanima.PMC1179934522KJ1247Kitaura KTakamatsu TKozono TItoh YMoriyama HWu CFInoue MNArai HfalseInfection pattern of male-killing viruses alters phenotypes in the tea tortrix moth Homona magnanima.Male-killing is a microbe-induced reproductive manipulation in invertebrates whereby male hosts are eliminated during development. In the tea tortrix moth Homona magnanima, Osugoroshi viruses 1‒3 (OGVs), belonging to Partitiviridae induce male-killing. The infection patterns of OGVs are diverse; however, how the influence of these patterns of host phenotypes remains largely unknown. Using field-collected larvae, we established a OGV1 and OGV3 double-infection line, in addition to a triple-infection line, and examined the dsRNA segments, purified viral proteins, OGV density, and host phenotypes. PCR analysis demonstrated that the triple-infection line lost one dsRNA segment, whereas the double-infection line lost eight segments, including one RNA-dependent RNA polymerase (RdRp) gene. LC-MS 2025-01-01T00:00:00Z2025 Feb2026-06-16T07:11:31.651Z2026-06-16T03:10:09.392ZS-EPMC117993453972569110.1038/s41437-024-00741-x