{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mirzadeh Azad F"],"funding":["Medical Research Council"],"pagination":["eadg7997"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11801372"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(33)"],"pubmed_abstract":["Understanding mechanisms of epigenetic regulation in embryonic stem cells (ESCs) is of fundamental importance for stem cell and developmental biology. Here, we identify <i>Spic</i>, a member of the ETS family of transcription factors (TFs), as a marker of ground state pluripotency. We show that <i>Spic</i> is rapidly induced in ground state ESCs and in response to extracellular signal-regulated kinase (ERK) inhibition. We find that SPIC binds to enhancer elements and stabilizes NANOG binding to chromatin, particularly at genes involved in choline/one-carbon (1C) metabolism such as <i>Bhmt</i>, <i>Bhmt2</i>, and <i>Dmgdh</i>. Gain-of-function and loss-of-function experiments revealed that <i>Spic</i> controls 1C metabolism and the flux of <i>S</i>-adenosyl methionine to <i>S</i>-adenosyl-L-"],"journal":["Science advances"],"pubmed_title":["&lt;i&gt;Spic&lt;/i&gt; regulates one-carbon metabolism and histone methylation in ground-state pluripotency."],"pmcid":["PMC11801372"],"funding_grant_id":["MR/X008517/1"],"pubmed_authors":["Wingert V","Atlasi Y","Mills K","Stunnenberg HG","Longley DB","Mirzadeh Azad F","Struys EA","Hannibal L","Jansen JH"],"additional_accession":[]},"is_claimable":false,"name":"&lt;i&gt;Spic&lt;/i&gt; regulates one-carbon metabolism and histone methylation in ground-state pluripotency.","description":"Understanding mechanisms of epigenetic regulation in embryonic stem cells (ESCs) is of fundamental importance for stem cell and developmental biology. Here, we identify <i>Spic</i>, a member of the ETS family of transcription factors (TFs), as a marker of ground state pluripotency. We show that <i>Spic</i> is rapidly induced in ground state ESCs and in response to extracellular signal-regulated kinase (ERK) inhibition. We find that SPIC binds to enhancer elements and stabilizes NANOG binding to chromatin, particularly at genes involved in choline/one-carbon (1C) metabolism such as <i>Bhmt</i>, <i>Bhmt2</i>, and <i>Dmgdh</i>. Gain-of-function and loss-of-function experiments revealed that <i>Spic</i> controls 1C metabolism and the flux of <i>S</i>-adenosyl methionine to <i>S</i>-adenosyl-L-","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-04T23:59:41.743Z","creation":"2025-04-04T23:59:41.743Z"},"accession":"S-EPMC11801372","cross_references":{"pubmed":["37595034"],"doi":["10.1126/sciadv.adg7997"]}}