{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu XM"],"funding":["Howard Hughes Medical Institute","Basic Research Program of Jiangsu Province","Foundation for the National Institutes of Health","National Natural Science Foundation of China","NCI NIH HHS"],"pagination":["559"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11803071"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["79(11)"],"pubmed_abstract":["Transcriptional programming plays a key role in determining the cell state. Timely reconfiguration of chromatin structure and attenuation of pluripotent genes are required for efficient embryonic stem cell (ESC) differentiation. Here, we identify METTL3, a core N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) catalyzing enzyme, as a crucial modulator of dynamic transcription and chromatin accessibility upon ESC-derived cardiac differentiation. Genome-wide analysis of chromatin-associated RNAs revealed that depletion of METTL3 failed to dramatically attenuate the transcription of pluripotent genes, as well as activate nascent cardiomyocyte-specific transcripts upon differentiation. Consistently, ATAC-seq analysis showed that loss of METTL3 markedly attenuated the dynamic alteration of chromat"],"journal":["Cellular and molecular life sciences : CMLS"],"pubmed_title":["METTL3 modulates chromatin and transcription dynamics during cell fate transition."],"pmcid":["PMC11803071"],"funding_grant_id":["BK20211219","R21CA227917","32070868","55108556","R21 CA227917","81974527","BK20190533"],"pubmed_authors":["Liu XM","Qian SB","Wang S","Mao Y","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"METTL3 modulates chromatin and transcription dynamics during cell fate transition.","description":"Transcriptional programming plays a key role in determining the cell state. Timely reconfiguration of chromatin structure and attenuation of pluripotent genes are required for efficient embryonic stem cell (ESC) differentiation. Here, we identify METTL3, a core N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) catalyzing enzyme, as a crucial modulator of dynamic transcription and chromatin accessibility upon ESC-derived cardiac differentiation. Genome-wide analysis of chromatin-associated RNAs revealed that depletion of METTL3 failed to dramatically attenuate the transcription of pluripotent genes, as well as activate nascent cardiomyocyte-specific transcripts upon differentiation. Consistently, ATAC-seq analysis showed that loss of METTL3 markedly attenuated the dynamic alteration of chromat","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-04T01:35:59.526Z","creation":"2025-04-04T01:35:59.526Z"},"accession":"S-EPMC11803071","cross_references":{"pubmed":["36266520"],"doi":["10.1007/s00018-022-04590-x"]}}