biostudies-literatureXie FResearch Project of Jiangsu Commission of HealthNational Natural Science Foundation of ChinaSocial Development Foundation of ZhenjiangNatural Science Foundation of Jiangsu Province215https://www.ebi.ac.uk/biostudies/studies/S-EPMC11806540biostudies-literatureUnknown25(1)<h4>Background</h4>The homeobox (HOX) genes especially for HOXA cluster play crucial roles in leukemogenesis. HOXA overexpression caused by genetic alterations, such as KMT2A rearrangements, NUP98- fusions and FLT3-ITD mutations, is frequently identified in AML. However, very few studies determined the DNA methylation-mediated epigenetic regulation of the HOXA cluster genes in AML.<h4>Methods</h4>We systematically first screened the prognostic value of HOXA cluster genes methylation in AML from The Cancer Genome Atlas (TCGA) datasets. Afterwards, the candidate prognosis-related gene HOXA9 were selected for clinical relevance analysis and were further validated in another independent cohort from our research center.<h4>Results</h4>The methylation of HOXA9, among HOXA cluster genes, negativeBMC cancerIdentification of HOXA9 methylation as an epigenetic biomarker predicting prognosis and guiding treatment choice in acute myeloid leukemia.PMC1180654082100183M2022123SH202300982270179BK2022128782300164BK20230296SH2022027Qian JXie FZhao YJWang YZhou JDZhang XLXu ZJZhang TJQiao LfalseIdentification of HOXA9 methylation as an epigenetic biomarker predicting prognosis and guiding treatment choice in acute myeloid leukemia.<h4>Background</h4>The homeobox (HOX) genes especially for HOXA cluster play crucial roles in leukemogenesis. HOXA overexpression caused by genetic alterations, such as KMT2A rearrangements, NUP98- fusions and FLT3-ITD mutations, is frequently identified in AML. However, very few studies determined the DNA methylation-mediated epigenetic regulation of the HOXA cluster genes in AML.<h4>Methods</h4>We systematically first screened the prognostic value of HOXA cluster genes methylation in AML from The Cancer Genome Atlas (TCGA) datasets. Afterwards, the candidate prognosis-related gene HOXA9 were selected for clinical relevance analysis and were further validated in another independent cohort from our research center.<h4>Results</h4>The methylation of HOXA9, among HOXA cluster genes, negative2025-01-01T00:00:00Z2025 Feb2025-04-04T01:34:01.225Z2025-04-04T01:34:01.225ZS-EPMC118065403992062410.1186/s12885-025-13633-y