biostudies-literatureGonzalez-Gonzalez AInstituto de Salud Carlos IIINCI NIH HHSMinisterio de Economía y CompetitividadNational Institute of Health (US)Instituto de Investigación Marqués de Valdecilla50https://www.ebi.ac.uk/biostudies/studies/S-EPMC11806587biostudies-literatureUnknown16(1)<h4>Background</h4>Osteoporosis (OP), characterized by reduced bone mass and mineral density, is a global metabolic disorder that severely impacts the quality of life in affected individuals. Although current pharmacological treatments are effective, their long-term use is often associated with adverse effects, highlighting the need for safer, more sustainable therapeutic strategies. This study investigates the pro-osteogenic and anti-resorptive potential of the secretome from Smurf1-silenced mesenchymal stem cells (MSCs) as a promising cell-free therapy for bone regeneration.<h4>Methods</h4>Conditioned media (CM) from Smurf1-silenced rat (rCM-Smur1) and human MSCs (hCM-Smurf1) was collected and analyzed. Pro-osteogenic potential was assessed by measuring in vitro mineralization in human aStem cell research & therapyParacrine activity of Smurf1-silenced mesenchymal stem cells enhances bone regeneration and reduces bone loss in postmenopausal osteoporosis.PMC11806587PID2021-127493OB-C21PREVAL 20/01R37-CA251763R01 CA209882PI22/0264PREVAL19/02R01-CA209882NEXT-VALAlvarez-Iglesias IPerez-Campo FMPerez-Nunez MIAlfonso-Fernandez AReyes RGarcia-Sanchez DDotta MRodriguez-Rey JCDelgado-Calle JDiaz-Rodriguez PBolado-Carrancio AGonzalez-Gonzalez AfalseParacrine activity of Smurf1-silenced mesenchymal stem cells enhances bone regeneration and reduces bone loss in postmenopausal osteoporosis.<h4>Background</h4>Osteoporosis (OP), characterized by reduced bone mass and mineral density, is a global metabolic disorder that severely impacts the quality of life in affected individuals. Although current pharmacological treatments are effective, their long-term use is often associated with adverse effects, highlighting the need for safer, more sustainable therapeutic strategies. This study investigates the pro-osteogenic and anti-resorptive potential of the secretome from Smurf1-silenced mesenchymal stem cells (MSCs) as a promising cell-free therapy for bone regeneration.<h4>Methods</h4>Conditioned media (CM) from Smurf1-silenced rat (rCM-Smur1) and human MSCs (hCM-Smurf1) was collected and analyzed. Pro-osteogenic potential was assessed by measuring in vitro mineralization in human a2025-01-01T00:00:00Z2025 Feb2025-04-04T02:43:33.111Z2025-04-04T02:43:33.111ZS-EPMC118065873992082410.1186/s13287-025-04165-0