biostudies-literatureYu SKey specialties for Joint Logistics MedicineQi-hang Foundation of Fujian Medical University38https://www.ebi.ac.uk/biostudies/studies/S-EPMC11806595biostudies-literatureUnknown25(1)<h4>Background</h4>Gastric cancer (GC) is one of the most common malignant tumors in the digestive system. However, the development of its targeted therapies has been slow. Therefore, exploring the mechanisms of malignant behavior of GC is key to developing their treatment methods. Pregnancy-associated plasma protein-A(PAPPA) is thought to play an important role in the occurrence and progression of cancer, yet its significance in the development of GC has not been reported.<h4>Methods</h4>Bioinformatics analysis elucidated PAPPA's expression in GC and its prognostic significance. The study correlated PAPPA expression with immune infiltration and signaling pathways. Cellular assays, including CCK-8, Western blotting, and flow cytometry, were utilized to examine PAPPA's role in gastric canceCancer cell internationalOverexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro investigations.PMC11806595LQZD-PW2021QH1327Qiu JWu JYu SChen JZheng SLin HChen YChen HWu WWang YYu XZhao HfalseOverexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro investigations.<h4>Background</h4>Gastric cancer (GC) is one of the most common malignant tumors in the digestive system. However, the development of its targeted therapies has been slow. Therefore, exploring the mechanisms of malignant behavior of GC is key to developing their treatment methods. Pregnancy-associated plasma protein-A(PAPPA) is thought to play an important role in the occurrence and progression of cancer, yet its significance in the development of GC has not been reported.<h4>Methods</h4>Bioinformatics analysis elucidated PAPPA's expression in GC and its prognostic significance. The study correlated PAPPA expression with immune infiltration and signaling pathways. Cellular assays, including CCK-8, Western blotting, and flow cytometry, were utilized to examine PAPPA's role in gastric cance2025-01-01T00:00:00Z2025 Feb2025-04-05T09:40:48.035Z2025-04-05T09:40:48.035ZS-EPMC118065953992067610.1186/s12935-025-03650-z