biostudies-literatureTian YNational Natural Science Foundation of China57https://www.ebi.ac.uk/biostudies/studies/S-EPMC11806715biostudies-literatureUnknown16(1)<h4>Background</h4>Liver transplantation is the most effective treatment for end-stage liver disease. However, the shortage of donor livers has become a significant obstacle to the advancement of liver transplantation. Mesenchymal stem cells-derived exosomes (MSCs-Exo) have been extensively investigated in liver diseases. However, the underlying mechanisms of how they can protect organ donation after cardiac death (DCD) livers remain unclear.<h4>Methods</h4>In this study, an arterialized mouse non-heart-beating (NHB) liver transplantation model was used to investigate the effect of MSCs-Exo on NHB liver transplantation. The survival rates, histology, pro-inflammatory cytokine and chemokine expression, and underlying mechanisms were investigated.<h4>Results</h4>The infusion of MSCs-Exo reduStem cell research & therapyMesenchymal stem cells-derived exosomes attenuate mouse non-heart-beating liver transplantation through Mir-17-5p-regulated Kupffer cell pyroptosis.PMC11806715No. 82000619Jiang YYe NTian YJin MGao ZYan SfalseMesenchymal stem cells-derived exosomes attenuate mouse non-heart-beating liver transplantation through Mir-17-5p-regulated Kupffer cell pyroptosis.<h4>Background</h4>Liver transplantation is the most effective treatment for end-stage liver disease. However, the shortage of donor livers has become a significant obstacle to the advancement of liver transplantation. Mesenchymal stem cells-derived exosomes (MSCs-Exo) have been extensively investigated in liver diseases. However, the underlying mechanisms of how they can protect organ donation after cardiac death (DCD) livers remain unclear.<h4>Methods</h4>In this study, an arterialized mouse non-heart-beating (NHB) liver transplantation model was used to investigate the effect of MSCs-Exo on NHB liver transplantation. The survival rates, histology, pro-inflammatory cytokine and chemokine expression, and underlying mechanisms were investigated.<h4>Results</h4>The infusion of MSCs-Exo redu2025-01-01T00:00:00Z2025 Feb2025-04-05T09:41:00.055Z2025-04-05T09:41:00.055ZS-EPMC118067153992084410.1186/s13287-025-04169-w