{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Szabo M"],"funding":["University of Debrecen Program for Scientific Publication","University Research Scholarship Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund","National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation","János Bolyai Research Scholarship of the Hungarian Academy of Sciences","Hungarian National Research, Development and Innovation Office"],"pagination":["889"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11816637"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(3)"],"pubmed_abstract":["Due to the lack of measurement techniques suitable for examining compartments of intact, living cells, membrane biophysics is almost exclusively investigated in the plasma membrane despite the fact that its alterations in intracellular organelles may also contribute to disease pathogenesis. Here, we employ a novel, easy-to-use, confocal microscopy-based approach utilizing F66, an environment-sensitive fluorophore in combination with fluorescent organelle markers and quantitative image analysis to determine the magnitude of the molecular order-related dipole potential in the plasma membrane and intracellular organelles of various tumor and neural cell lines. Our comparative analysis demonstrates considerable intracellular variations of the dipole potential that may be large enough to modula"],"journal":["International journal of molecular sciences"],"pubmed_title":["Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells."],"pmcid":["PMC11816637"],"funding_grant_id":["EKÖP-24-2-DE-205","BO/00392/23","ANN133421","BO/00676/24","OTKA K132906","EKÖP-24-4-II-DE-74","OTKA FK143400","OTKA FK146740","OTKA K143071","OTKA K138075"],"pubmed_authors":["Nagy P","Zakany F","Panyi G","Cs Szabo B","Szabo M","Kurtan K","Kovacs T","Varga Z"],"additional_accession":[]},"is_claimable":false,"name":"Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells.","description":"Due to the lack of measurement techniques suitable for examining compartments of intact, living cells, membrane biophysics is almost exclusively investigated in the plasma membrane despite the fact that its alterations in intracellular organelles may also contribute to disease pathogenesis. Here, we employ a novel, easy-to-use, confocal microscopy-based approach utilizing F66, an environment-sensitive fluorophore in combination with fluorescent organelle markers and quantitative image analysis to determine the magnitude of the molecular order-related dipole potential in the plasma membrane and intracellular organelles of various tumor and neural cell lines. Our comparative analysis demonstrates considerable intracellular variations of the dipole potential that may be large enough to modula","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T11:58:41.689Z","creation":"2025-04-04T11:58:41.689Z"},"accession":"S-EPMC11816637","cross_references":{"pubmed":["39940660"],"doi":["10.3390/ijms26030889"]}}