biostudies-literatureMaranon PInstituto de Salud Carlos III1187-1202https://www.ebi.ac.uk/biostudies/studies/S-EPMC11821676biostudies-literatureUnknown99(3)Given the lack of accurate diagnostic methods of acetaminophen (APAP)-induced acute liver failure (ALF), the search for new biomarkers for its diagnosis is an urgent need. The aim of this study was to evaluate the role of bone morphogenetic protein 6 (BMP6) in APAP-induced ALF progression and its potential value as a biomarker of ALF. Hepatic and circulating BMP6 expression was assessed in APAP-treated mice and in serum samples from patients with APAP overdose. In addition, BMP6 expression and release was evaluated in hepatocytes after APAP exposure. BMP6 gene was silenced in Huh7 cells prior to APAP treatment and the culture medium (CM) was added to THP1 cells to evaluate the paracrine effects of hepatocyte BMP6 on APAP toxicity. Hepatic and serum BMP6 levels were increased in mice after Archives of toxicologyBMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity.PMC11821676CP19/00032PI19/00123PI22/01968Egea JGarcia-Garcia YDear JWValverde AMRey EGonzalez-Rodriguez AMaranon PGarcia-Monzon CIsaza SCRada PfalseBMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity.Given the lack of accurate diagnostic methods of acetaminophen (APAP)-induced acute liver failure (ALF), the search for new biomarkers for its diagnosis is an urgent need. The aim of this study was to evaluate the role of bone morphogenetic protein 6 (BMP6) in APAP-induced ALF progression and its potential value as a biomarker of ALF. Hepatic and circulating BMP6 expression was assessed in APAP-treated mice and in serum samples from patients with APAP overdose. In addition, BMP6 expression and release was evaluated in hepatocytes after APAP exposure. BMP6 gene was silenced in Huh7 cells prior to APAP treatment and the culture medium (CM) was added to THP1 cells to evaluate the paracrine effects of hepatocyte BMP6 on APAP toxicity. Hepatic and serum BMP6 levels were increased in mice after 2025-01-01T00:00:00Z2025 Mar2026-06-01T08:48:53.635Z2025-04-07T11:28:02.088ZS-EPMC118216763982745010.1007/s00204-024-03954-5